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Catalog Products » Protein Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » Protein L Resin

Protein L Resin


Description GenScript Protein L Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The highly purified protein L ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein L Resin is greater than 15 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Protein L, first isolated from the surface of bacterial species Peptostreptoccus magnus, binds immunoglobulin through κ light chain interaction. Protein L binds a wider range of Ig classes and subclasses than other antibody-binding proteins such as protein A or protein G. It can bind to all classes of Ig (i.e., IgG, IgM, IgA, IgE, and IgD) and also the single chain variable fragments (Scfv) and Fab fragments.
Note: High temperature heating is not recommended. The agarose melts above 65°C.

Cat. No.Product Name Volume supplied Resin content
L00239Protein L Resin (2.5ml)5 ml50% slurry

Storage:Store at 4°C, do NOT freeze.
Key Features
Key Features
  • Broad Ig binding spectrum
  • High binding capacity, more than 15 mg immunoglobulin per ml of resin
  • Cross-linked agarose media

Specifications
Ligand Highly purified protein L
Number of Ig binding sites per ligand5
MW of ligandApproximately 42 KD
PI of ligand4.57
Degree of substitution Approximately 2 mg protein L/ml settled resin
Static binding capacity >15 mg rabbit IgG/ml settled resin
Matrix spherical Agarose, 4% cross-linked
Average particle size 90 μm (45 - 165 μm)
Storage solution 1×PBS containing 20% ethanol
SanitizationWashing of the packed column with 70% ethanol
Storage & stability 18 months when stored unopened at 2 to 8 °C

Storage Store at 4°C
Protein L Resin
Protein L Resin


Zhang D., et al. Generation and Characterization of a Novel Recombinant Antibody against LMP1-TES1 of Epstein-Barr Virus Isolated by Phage Display. Viruses. 2013 Apr 22;5(4):1131-42.

De Oliveira SN., et al. A CD19/Fc fusion protein for detection of anti-CD19 chimeric antigen receptors. J Transl Med. 2013 Jan 29;11:23.

Chen R., et al. A Human Fab-Based Immunoconjugate Specific for the LMP1 Extracellular Domain Inhibits Nasopharyngeal Carcinoma Growth In Vitro and In Vivo. Mol Cancer Ther. 2012 Mar;11(3):594-603.

Kip Dudgeon, et al. General Strategy For The Generation Of Human Antibody Variable Domains With Increased Aggregation Resistance. Proc Natl Acad Sci. 2012 Jul 3;109(27):10879-84.

Rouet R., et al. Expression of high-affinity human antibody fragments in bacteria. Nat Protoc. 2012 Feb 2;7(2):364-73.

Safdari Y., et al. humMR1, a highly specific humanized single chain antibody for targeting EGFRvIII. Int Immunopharmacol. 2013Dec 25;18(2):304-310.

Wang X., et al. Anti-idiotypic antibody specific to GAD65 autoantibody prevents type 1 diabetes in the NOD mouse. PLoS One. 2012 7(2):e32515.

R Lindner, et al. Process development of periplasmatically produced single chain fragment variable against epidermal growth factor receptor in Escherichia coli. J Biotechnol. 2014Dec.;

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Cat. No. L00239
Size
Price
2.5 ml $145.00
 
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