Protein A/G MagBeads

GenScript Protein A/G MagBeads are ideal for small‐ scale antibody purification and immunoprecipitation (IP) of proteins, protein complexes or other antigens.

Price	$153.00
Cat. No.	L00277
Size	2 ml (0.5 ml Settled Beads) Buy in bulk
Quantity
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Description

Intended Use
GenScript Protein A/G MagBeads are ideal for small‐ scale antibody purification and immunoprecipitation (IP) of proteins, protein complexes or other antigens.

Principle
The sample containing antibody is added to the Protein A/G MagBeads. The antibody will bind to beads during a short incubation. Then the beads-bound antibody may be eluted from the beads by using a magnetic separation rack, or used for immunoprecipitation (IP). A cross-linking procedure may be needed before IP to prevent co-elution of the primary antibody. Magnetic separation eliminates the changes of micro tubes, minimizes the loss of sample and removes excessive steps of traditional centrifugation method.

Material Supplied
GenScript Protein A/G MagBeads are super paramagnetic beads of average 40 μm in diameter, covalently coated with recombinant Protein A/G. The beads are supplied as 25% slurry in 20% ethanol. The Protein A/G MagBeads have a binding capacity of more than 10 mg Human IgG per 1 ml settled beads (e.g. 4 ml 25% slurry).
Protein A/G is a genetically engineered protein (MW≈43 kDa) that combines the IgG binding sites of both Protein A and Protein G. 6× His‐ tag was attached to its N‐ terminal to facilitate the purification. The secreted Protein A/G contains four Fc‐ binding domains from Protein A and two from Protein G, making it a more universal tool to bind and purify immunoglobulins.

Storage and Stability
This product is stable until the expiration date stated on the COA, when stored unopened at 2-8°C. Do not freeze the product. Keep the MagBeads in liquid suspension during storage and all handling steps. Drying will cause loss of binding capacity and result in reduced performance. Resuspend the beads well before use. Be careful to avoid bacterial/fungal contamination.

For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.

Citations

1. Jiaqi Tang, et al. OsWRKY53-OsGT1 Module Regulates Rice Tiller Development and Is Involved in Fine-Tuning Strigolactone Signaling. Plant Biotechnology Journal. (2026-02)
2. Enyang Mei, et al. OsWRKY78 regulates panicle exsertion via gibberellin signaling pathway in rice. J Integr Plant Biol. (2024-03)
3. Shengjie Zhang, et al. NEAT1 repression by MED12 creates chemosensitivity in p53 wild-type breast cancer cells. FEBS J. (2024-02)
4. Peixia Tang , et al. CRIP1 involves the pathogenesis of multiple myeloma via dual-regulation of proteasome and autophagy. EBioMedicine. (2024-02)
5. Jing-Yi Zhou, et al. Discovery and identification of a novel small molecule BCL-2 inhibitor that binds to the BH4 domain. Acta Pharmacol Sin. (2022-08)
6. Guangxin Zhao, et al. OsASHL1 and OsASHL2, two members of the COMPASS-like complex, control floral transition and plant development in rice. J Genet Genomics. (2022-03)
7. Jingru Guo, et al. Fine-tuning of pathogenesis-related protein 1 (PR1) activity by the melatonin biosynthetic enzyme ASMT2 in defense response to cassava bacterial blight. J Pineal Res. (2021-12)
8. Jingru Guo, et al. Fine-tuning of pathogenesis-related protein 1 (PR1) activity by the melatonin biosynthetic enzyme ASMT2 in defense response to cassava bacterial blight. J Pineal Res. (2021-12)
9. Caroline Jane Ross, et al. Uncovering deeply conserved motif combinations in rapidly evolving noncoding sequences. Genome Biol. (2021-01)
10. Ren Y, et al. Oryza sativa mediator subunit OsMED25 interacts with OsBZR1 to regulate brassinosteroid signaling and plant architecture in rice. Journal of integrative plant biology. (2020-06)
11. Fang Y, et al. Epigenetic dysregulation of Mdr1b in the blood-testis barrier contributes to dyszoospermia in mice exposed to cadmium. Ecotoxicol Environ Saf. (2020)
12. Rom A, et al. Regulation of CHD2 expression by the Chaserr long noncoding RNA gene is essential for viability. Nat Commun. (2019)
13. RestrepoRicardo J, et al. Binge alcohol alters PNPLA3 levels in liver through epigenetic mechanism involving histone H3 acetylation. Alcohol. (2017-05)
14. Wang Chao, et al. Lotus japonicus clathrin heavy Chain1 is associated with Rho-Like GTPase ROP6 and involved in nodule formation. Plant Physiol. (2015)

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