Catalog Products » TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)
Manual
Manual
COAs

TUNEL Apoptosis Detection Kit (Paraffin-embedded Tissue Section, Biotin-labeled POD)

*This product has been discontinued!*
none
L00297
Ask us a question
Description


Description

The TUNEL Apoptosis Detection Kit for Paraffin-embedded Tissue Sections (Biotin labeled POD ) (Cat. No. L00297) is one of GenScript’s newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, biotinylated nucleotide is labeled at the DNA 3´-OH ends using the natural or recombinant terminal deoxynucleotidyl transferase (TdT or rTdT). Then, horseradish peroxidase-labeled streptavidin (streptavidin-HRP) is bound to these biotinylated nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-diaminobenzidine (DAB)-Solution, a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.
Product Introduction
Key Features
Key features

  • Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K, DAB-Solution and DNase I.
  • Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
  • Enhanced Specificity: The kit can stain apoptotic cells.
  • Enhanced Stability: The kit has improved stability than traditional TUNEL detection kit.
  • Streamlined Process: The entire procedure takes about three hours.
  • Increased Convenience: The results can be observed by light microscope.
  • High Veracity: The kit contains positive control reagent.
    		•
    Storage Store Streptavidin-HRP, DAB-A Solution, DAB-B Solution, DAB-C Solution at 4°C, and do not expose it to light. Store the rest of the kit at -20°C. It will remain stable for one year.
    Back

    Citations
    FangChengzhi, et al. Berberine ameliorates neonatal necrotizing enterocolitis by activating the phosphoinositide 3-kinase/protein kinase B signaling pathway. Exp Ther Med. (2018-04)
    YildizMustafa, et al. Changes in rat ovary with experimentally induced diabetes and the effects of lycopene on those changes. Rom J Morphol Embryol. (2016)
    Gato WE, et al. Time-Dependent Regulation of Apoptosis by AEN and BAX in Response to 2-Aminoanthracene Dietary Consumption. Toxicol Int. (2014)
    F Trea, et al. La Glisodin®, un extrait de melon, attÉnue l'apoptose des cardiomyocytes via la suppression du stress oxydant cardiaque au cours du diabÈte chronique expÉrimental. PhytothÉrapie. (2013-11)
    PK Manchanda, et al. Rac1 Is Required For Prkar1A-Mediated Nf2 Suppression In Schwann Cell Tumors. Oncogene. (2012-10)
    Bid HK, et al. Anti-angiogenic activity of a small molecule STAT3 inhibitor LLL12. PLoS One. (2012-04)
    Perin L, et al. Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis. PLoS One. (2010-02)