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| L00310 | |
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Description:Acridine orange is a fluorescent pigment, the detection wavelength of 488 nm excitation filter. Its blocking filter wavelength is 515 nm. With cell DNA and RNA-binding capacity gaps exist, it may issue a different color fluorescence, and DNA binding of less green fluorescence, and the amount of RNA-binding orange or orange red fluorescence. The dye is membrane permeability, through the membrane, so that nuclear DNA and RNA stain. Therefore, the observed under fluorescence microscope, acridine orange through the normal cell membrane to the nucleus was green or yellow-green uniform fluorescence; In apoptotic cells as a result of chromatin condensation or fracture fragments ranging from the size, formation of apoptotic bodies. Acridine orange stain to the infected dense green fluorescent yellow or yellowish green debris particles; The yellow fluorescence cell necrosis weaken or even disappear. Acridine orange and AO often combined bromide啶EB double staining, only with death by EB cells thus producing orange fluorescence, which can be divided into normal cells, apoptotic cells and necrotic cells. |
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