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Cell Apoptosis PI Detection Kit

*This product has been discontinued!*
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L00311
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Description

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Fluorescent labeling is fundamental to apoptosis research. It can be used to assay the morphological and biochemical changes involved in apoptosis in normal, apoptotic, and dead cells.
GenScript's Cell Apoptosis PI Detection Kit (Cat.No.L00311) provides a rapid and convenient assay for apoptosis based upon fluorescent detection. The kit includes ready-to-use propidium iodide (PI), a DNA-specific red-fluorescence dye (excitation/emission maxima ≈535/617 nm). Propidium iodide is only permeant to dead cells. Under fluorescent microscopy, normal cells are not stained, while dead cells show strong red fluorescence. The intensity of the fluorescence of apoptotic cells is between dead cells and normal cells. Under flow cytometry, DNA in the cells is degraded by endogenous nuclease activity and diffused out of cells as part of apoptosis. A highly definable sub-G1 peak occurs and is easily quantified by PI. Cells can then be sorted and further analyzed by microscopy to verify the presence of apoptotic cells. The change in DNA of the apoptotic cells is then assayed by flow cytometry.
Product Introduction
Key Features
Key features:

  • Extra Convenience: The kit is supplied ready to use.
  • Simplified Procedure: The kit is quick and easy to use
  • Strong Versatility: The kit can directly analyze normal and apoptotic cells by flow cytometry, fluorescence microscopy, or fluorescent photometry.
Storage This kit remains stable for at least one year if stored at 4°C and protected from light.
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Citations
Gunasekaran S, et al. p-Methoxycinnamic acid, an active phenylpropanoid induces mitochondrial mediated apoptosis in HCT-116 human colon adenocarcinoma cell line. Environ Toxicol Pharmacol. (2015-09)
SivagamiGunasekaranKarthikkumarVenkatachalamNaliniNamasivayam, et al. p-Methoxycinnamic acid, an active phenylpropanoid. Environmental Toxicology and Pharmacology. (2015)