|Description|| GenScript Streptavidin Resin is an affinity chromatography medium designed for easy,
one-step purification of biotinylated peptides, antibodies, lectins, etc
from samples. The purified recombinant streptavidin is covalently coupling to 4%
highly cross-linked agarose. The
coupling is optimized to give high binding capacity for biotinylated molecules. The total
binding capacity of Streptavidin Resin is more
than 120 nmol of D-Biotin/ml
settled resin. Table 1 lists the characteristics of Streptavidin Resin.
Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Streptavidin binds 4 moles of biotin per mole of protein with an extremely high affinity. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point of 6.5 (vs 10 for avidin) close to where most useful biological interactions occur. As a result, streptavidin frequently exhibits much lower non-specific binding than avidin does.
|Key Features||Characteristics of Streptavidin Resin
|Storage||2 - 8°C; DO NOT FREEZE|
|Note||High temperature heating is not recommended. The agarose melts above 65°C.|
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