Catalog Products » Protein Isolation and Purification » Protein Purification Resins » Streptavidin Resin

Streptavidin Resin

GenScript Streptavidin Resin is an affinity chromatography medium designed for easy, one-step purification of biotinylated peptides, antibodies, lectins, etc from samples. The purified recombinant streptavidin is covalently coupling to 4% highly cross-linked agarose. The coupling is optimized to give high binding capacity for biotinylated molecules. The total binding capacity of Streptavidin Resin is more than 120 nmol of D-Biotin/ml settled resin. Table 1 lists the characteristics of Streptavidin Resin.
L00353
$152.90

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Description

GenScript Streptavidin Resin is an affinity chromatography medium designed for easy, one-step purification of biotinylated peptides, antibodies, lectins, etc from samples. The purified recombinant streptavidin is covalently coupling to 4% highly cross-linked agarose. The coupling is optimized to give high binding capacity for biotinylated molecules. The total binding capacity of Streptavidin Resin is more than 120 nmol of D-Biotin/ml settled resin. Table 1 lists the characteristics of Streptavidin Resin.


Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Streptavidin binds 4 moles of biotin per mole of protein with an extremely high affinity. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point of 6.5 (vs 10 for avidin) close to where most useful biological interactions occur. As a result, streptavidin frequently exhibits much lower non-specific binding than avidin does.

Note

High temperature heating is not recommended. The agarose melts above 65°C.

Overview
Product Information
Cat. No.Product NameVolume supplied Resin content 
L00353Streptavidin Resin (5 ml)10 ml50% slurry
Key Features Characteristics of Streptavidin Resin
Matrix sphericalagarose, 4% cross-linked
LigandRecombinant Streptavidin
Total binding capacity
> 120 nmol of D-Biotin/ml settled resin
Average particle size90 μm (45 - 165 μm)
Storage solution1X PBS containing 20% ethanol 

Properties
Storage 2 - 8°C; DO NOT FREEZE

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