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Mouse NGF ELISA Kit

*This product has been discontinued!*
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L00395
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Description


Kit Description

GenScript’s Mouse NGF ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody specific for mouse NGF is precoated onto each 96-well plate. Standards and samples are added to the wells and any NGF present is bound by the immobilized antibody. After any unbound substance is washed away with PBS or TBS buffer, a biotinylated detection polyclonal antibody specific for mouse NGF is added to the wells. Following a wash to remove any unbound biotinylated antibody, avidin-biotin-peroxidase complex substrate, or HRP substrate, TMB is used to visualize the enzymatic coloration reaction. Once catalyzed by HRP, TMB turns first blue and then yellow after coming into contact with the acidic stop solution. The density of the yellow pigment is proportional to the quantity of mouse NGF captured.
Product Introduction
Key Features
Key Features

  • Easy to perform: The 96-well plate precoated with antibody has simple and rapid procedure to perform.
  • High sensitivity: The kit can assay the concentration of NGF more than 1 pg/ml.
  • Large detection range: The kit can detect NGF from 31.2 pg/ml to 2000 pg/ml.
  • Super specificity: There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.
  • Reproducible results: The kit produces highly reproducible results.
    • Storage This kit remains stable for at least eight months if stored at -20°C and for at least four months if stored at 4°C. Store any kit meant for frequent use at 4°C. Avoid multiple thawing and freezing cycles.
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    Applications

    Applications

    This kit is designed for quantitive detection of mouse NGF in sera, plasma, body fluids, tissue lysates, and cell culture supernates.
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