Ultra Protein A Resin

*This product has been discontinued!*

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Cat. No.	L00400
Size	Buy in bulk

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Overview Properties

Description

GenScript Ultra Protein A Resin is an affinity chromatography medium designed for easy, one-step and large-scale purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Compared to Protein A Resin (GenScript, L00210), the recombinant protein A ligand is more densely coupled to 4% highly cross-linked agarose, resulting in much higher static binding capacity – 30 to 40 mg Rabbit IgG/ml settled resin. The dynamic binding capacity of Ultra Protein A Resin will vary depending on several factors such as target antibody, flow rate etc.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.

Note: High temperature heating is not recommended. The agarose melts above 65°C.

Total Volume:

5 ml settled resin (10 ml 50% slurry)

Storage:

Store at 4°C, do NOT freeze.

Overview

Key Features

Key Features

Strong Versatility: This product has a broad IgG binding spectrum.
High Binding Capacity: The capacity of Ultra Protein A Resin is 30-40 mg rabbit IgG per ml of resin

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Properties

Storage & Stability

Store GenScript Protein A Resin-Higher Binding Capacity in binding buffer A containing 20% ethanol at 2°C to 8°C. Do not freeze.

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