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High-Affinity Iodoacetyl Resin

Description GenScript High-Affinity Iodoacetyl Resin is produced by covalently coupling a derivative of iodoacetic acid to 4% cross-linked agarose beads. It is designed for easy and fast immobilization of sulfhydryl-containing peptides, proteins and other ligands. The resin reacts specifically with free sulfhydryls to form stable thioether linkage. The resin is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification. 1 ml settled resin can be coupled with more than 1 mg sulfhydryl-containing peptides of 7 amino-acids.
The High-Affinity Antibody Purification Kit contains High-Affinity Iodoacetyl Resin and all the necessary buffers needed for antibody purification.
Note: High temperature heating is not recommended. The agarose melts above 65°C.

Product Details
Cat. No.Product Name Volume supplied Resin content
L00403High-Affinity Iodoacetyl Resin (10 ml)20 ml50% slurry
Key Features
Key Features:
  • High stability: The resin is stable at a broad pH range from 5 to 10.
  • High binding capacity: More than 3 mg Goat IgG/ml resin.
  • Easy to use: The kit provides all the necessary buffers.

Binding Capacity More than 3 mg Goat IgG/ml resin.
Ligand The derivative of Iodoacetic acid
pH Stability pH 5 -10
Support Matrix 4% cross-linked agarose, spherical beads
Mean Particle Size 90 μm
Storage 2°C to 8°C
Shelf Life 18 months

Storage Store High-Affinity Iodoacetyl Resin in 1 M sodium chloride at 4°C in dark, do not freeze. Buffers should be stored at 4°C. All the other materials are stored at room temperature.

Hu J., et al. The Bphi008a gene interacts with the ethylene pathway and transcriptionally regulates MAPK genes in the response of rice to brown planthopper feeding. Plant Physiol. 2011 Jun;156(2):856-72.

Calligaris D., et al. Tyrosine-dependent capture of CAP-Gly domain-containing proteins in complex mixture by EB1 C-terminal peptidic probes. J Proteomics. 2012 Jun 27;75(12):3605-16.

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Cat. No. L00403
10 ml $150.00