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High-Affinity Antibody Purification Kit

Description GenScript High-Affinity Iodoacetyl Resin is produced by covalently coupling a derivative of iodoacetic acid to 4% cross-linked agarose beads. It is designed for easy and fast immobilization of sulfhydryl-containing peptides, proteins and other ligands. The resin reacts specifically with free sulfhydryls to form stable thioether linkage. The resin is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification. 1 ml settled resin can be coupled with more than 1 mg sulfhydryl-containing peptides of 7 amino-acids.
The High-Affinity Antibody Purification Kit contains High-Affinity Iodoacetyl Resin and all the necessary buffers needed for antibody purification.
Store at 4°C, do NOT freeze.
Key Features
Key Features:
  • High stability: The resin is stable at a broad pH range from 5 to 10.
  • High binding capacity: 1 ml resin can bind more than 3 mg Goat IgG.
  • Easy to use: The kit provides all the necessary buffers.

  • Components:
    High-Affinity Iodoacetyl Resin 5 ml (10 ml of 50% slurry)
    Empty Column3
    5X Coupling Buffer30 ml
    Block Buffer (Solid)10 ml (10 ml of 1X Coupling Buffer to the solid)
    Storage Buffer20 ml
    5X Binding/Wash Buffer70 ml
    Elution Buffer70 ml
    Neutralizing Buffer15 ml

    Storage Store High-Affinity Iodoacetyl Resin in 1 M sodium chloride at 4°C in dark, do not freeze. Buffers should be stored at 4°C. All the other materials are stored at room temperature.

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    Cat. No. L00404
    1 kit $139.00