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Human IL-8 ELISA Detection Kit

Human IL-8 ELISA Detection Kit

Human IL-8 ELISA Detection Kit

GenScript Human IL‐8 ELISA Detection Kit can be applied for quantitative detection of Human IL‐8 concentrations in plasma, blood and cell culture supernatant samples.
L00417
$378.00

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Overview
Product Description Interleukin‐8 (IL‐8), a member of a family of proinflammatory cytokines, is a neutrophil chemotactic cytokine that can signal through the CXCR1 and CXCR2 receptor. It is proinflammatory and primarily mediates the activation and migration of neutrophils from peripheral blood into the sites of inflammation, injury, or infection in the tissue. IL‐8 is involved in a wide variety of physiological and pathological processes, including host defense against bacterial infection, bronchiolitis, arteriosclerosis, autoimmune disorders of skin, and angiogenesis‐dependent disorders such as tumor growth, and wound repair. IL‐8 is produced by stimulated monocytes, leukocytic cells, non‐leukocytic somatic cells and tumor cells.
GenScript Human IL‐8 ELISA Detection Kit can be applied for quantitative detection of Human IL‐8 concentrations in plasma, blood and cell culture supernatant samples. The assay utilizes solid sandwich enzyme immunoassay in which two anti IL‐8 monoclonal antibodies function as bridge linkers. IL‐8 Capture Plate, which is a 96‐well plate pre‐coated with one anti IL‐8 monoclonal antibody, is used for capture of IL‐8 in serial IL‐8 standard solutions and sample solutions. IL‐8 Detection Reagent, which is a horseradish peroxidase (HRP) enzyme conjugated to the other anti IL‐8 monoclonal antibody, is used for enzyme reaction. In the assay, IL‐8 molecular is fixed between the Capture Plate and the Detection Reagent to form a sandwich complex vial both antibodies. Other unbound moleculars can be removed by several washings. The IL‐8 Detection Reagent reacts with TMB Solution that provides absorbance value, which can be obtained by Microplate reader. In optimized test condition, each absorbance value is indicated to the individual IL‐8 amount in solution. The IL‐8 standards of known concentration and corresponding absorbance values are used to form a standard curve. With the standard curve, IL‐8 amount present in the unknown sample is calculated by transforming its absorbance value.
Key Features Sensitivity: 2.8 pg/ml
Detection Range: 25~1600 pg/ml
Test Samples: serum, plasma and cell culture supernatant
Time Saving Protocol ‐ complete test in 2.5 hours
Ready‐to‐use ELISA plate with fast & simple sample preparation
Lot‐to‐lot consistency guaranteed by strict procedure

Product Introduction
Sensitivity The minimum detectable dose (MDD) of IL‐8 was 2.8 pg/ml. MDD is defined as the concentration corresponding to a signal three standard deviations above the mean of the zero standard.
Storage The reagents in the kit are stable for 12 months when stored at 2‐8°C. It should not be used beyond the expiration date. Do not freeze the kit.
Kit Contents
Components Quantity Part.No
IL‐8 Capture Plate 1 plate (8 wells x 12 strips) 417‐80
IL‐8 Detection Reagent 15 ml 417‐30
IL‐8 Standard 2 vials 417‐10
Sample Diluent 30 ml 417‐60
Assay Diluent 15 ml 417‐90
20× Wash Solution 40 ml B-A1-70
TMB Solution 12 ml B-A1-40
Stop Solution 6 ml B-A1-50
Adhesive Plate Cover 2 pieces N/A

Examples
  • Human IL-8 ELISA Detection Kit
    • Human IL-8 ELISA Detection Kit

  • Human IL-8 ELISA Detection Kit
    • Human IL-8 ELISA Detection Kit


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.