Protein A affinity chromatography is widely used for antibody purification. However, the leakage of Protein A, though covalently conjugated to chromatography supports, posts a big problem for antibody based pharmaceutical industry where false results could be generated in immunological assays from contaminated Protein A.
GenScript Protein A ELISA Kit
is developed for accurate quantification of the leakage of native Protein A, recombinant Protein A variants and MabSelect SuRe TM
in the presence or absence of antibodies. The kit offers pharmaceutical users a sensitive assay to measure mini amount of Protein A in therapeutic antibody products. It also facilitates Protein A resin manufacturers in monitoring leaching characteristics of their resins under specific conditions.
Accurate detection of 20 pg/ml Protein A and 50 pg/ml MabSelect SuRe TM
in antibody preps
Broad-range recongnition of native Protein A, recombinant Protein A variants and MabSelect SuRe
Low level deviation - Intra-assay
<5%, Inter-assay <10%
- High accuracy/recovery within 85-115%
Ready-to-use ELISA plate with fast & simple samples preparation
- Time-saving protocol - complete assay in less than 2 hours
Exceptional lot-to-lot consistency ensured by high-quality monoclonal antibodies
Wide applications for the quantification of Protein A contaminant and leaching
The kit is stable for 12 months if stored at 2-8 ℃.
Do NOT freeze any component of the kit.