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Catalog Products » Protein Analysis & Purification » Protein Isolation and Purification » Protein Purification Resins » Anti-DYKDDDDK G1 Affinity Resin

Anti-DYKDDDDK G1 Affinity Resin

DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. GenScript Anti-DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK-tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. The DYKDDDDK-tagged protein in the cell lysate can specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin. Nonspecific bindings are eliminated by stringent washing steps, and the target protein is finally eluted with high recovery rate. Table 1 lists the main characteristics of Anti-DYKDDDDK G1 Affinity Resin.

Key Features

Key Features
  • High DYKDDDDK binding capacity and ultralow levels of non-specific binding
  • Multiple elution methods for diverse protein characteristics and different requirements
  • Used for purification and immunoprecipitation of DYKDDDDK-tagged protein or protein complex

Characteristics:
Product content 50% settled resin in TBS with 0.02% sodium azide

Matrix

4% cross-linked agarose

Average bead size

90 μm

Ligand

Mouse monoclonal antibody against DYKDDDDK tag, clone G1

Binding capacity

Approximately 1 mg DYKDDDDK-tagged protein per ml settled resin

Storage and stability

Store at 2-8 °C for up to 12 months. Do not freeze the resin.

Resin reuse

The resin can be recycled for at least 4 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.

Elution method

Acid, alkaline, neutral, peptide competitive or PAGE loading buffer

NOTE:Elution with SDS-PAGE loading buffer causes denaturation of the G1 antibody.

Reagents compatibility

Compatible with commonly used reagents at certain concentrations


Comparison between Anti-DYKDDDDK IP Resin and Anti-DYKDDDDK G1 Affinity Resin.
Anti-DYKDDDDK G1 Affinity Resin (L00432) Anti-DYKDDDDK IP Resin (L00425)
Immunoprecipitation (IP)
Purification n/a
Elution Alkaline elution n/a
Alkalescent elution n/a
Acid elution n/a
Neutral elution n/a
Competitive elution n/a
SDS-PAGE loading synthesize buffer
Regeneration 4-10 times not recommend
Binding volume large scale mini-volume (<1ml)

Cat. No. Product Name Quantity Price
RP10586-1
DYKDDDDK Peptide - 1 mg
$34.00
L00455W
DYKDDDDK Tag Antibody Plate (White, 96-well) - 5 plates
$229.00
L00455C
DYKDDDDK Tag Antibody Plate (Clear, 8X12 strip) - 5 plates
$229.00
L00455B
DYKDDDDK Tag Antibody Plate (Black, 96-well) - 5 plates
$229.00
L00432-1
Anti-DYKDDDDK G1 Affinity Resin - 1 ml Resin (Total 2 ml)
$281.00
L00432-25
Anti-DYKDDDDK G1 Affinity Resin - 25 ml Resin (Total 50 ml)
$2591.00
L00432-5
Anti-DYKDDDDK G1 Affinity Resin - 5 ml Resin (Total 10 ml)
$663.00
L00432-10
Anti-DYKDDDDK G1 Affinity Resin - 10 ml Resin (Total 20 ml)
$1193.00
L00425
Anti-DYKDDDDK IP Resin - 1 ml Resin (Total 2 ml)
$199.00
A01811-100
THE™ DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse - 100 μg
$167.00
A01810-100
THE™ DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse - 100 μg
$167.00
A01809-100
THE™ DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse - 100 μg
$167.00
A01632
THE™ DYKDDDDK Tag Antibody [FITC], mAb, Mouse - 100 μg
$149.00
A01429-100
THE™ DYKDDDDK Tag Antibody [Biotin], mAb, Mouse - 100 μg
$149.00
A01428-100
THE™ DYKDDDDK Tag Antibody [HRP], mAb, Mouse - 100 μg
$149.00
A00187-100
THE™ DYKDDDDK Tag Antibody, mAb, Mouse - 100 μg
$139.00
A00187-200
THE™ DYKDDDDK Tag Antibody, mAb, Mouse - 200 μg
$256.00
A00170-40
DYKDDDDK-tag Antibody, pAb, Rabbit - 40 μg
$99.00
 
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Madison T Wright, et al. Thyroglobulin interactome profiling defines altered proteostasis topology associated with thyroid dyshormonogenesis. Mol Cell Proteomics. (2020-11)

Hessman CL, et al. YB-1 Interferes with TNFα-TNFR Binding and Modulates Progranulin-Mediated Inhibition of TNFα Signaling. International Journal of Molecular Sciences. (2020-09)

Ruinan Shen, et al. Upregulation of RIN3 induces endosomal dysfunction in Alzheimer's disease. Transl Neurodegener. (2020-06-01)

Qiao S, et al. Interconversion between Anticipatory and Active GID E3 Ubiquitin Ligase Conformations via Metabolically Driven Substrate Receptor Assembly. Molecular Cell. (2020)

Ferrari R, et al. TFIIIC Binding to Alu Elements Controls Gene Expression via Chromatin Looping and Histone Acetylation. Mol Cell. (2020)

Jialin Zhang, et al. Pathogenicity of porcine deltacoronavirus (PDCoV) strain NH and immunization of pregnant sows with an inactivated PDCoV vaccine protects 5-day-old neonatal piglets from virulent challenge. Transbound Emerg Dis. (2019-09-01)

SpradlinJessica N, et al. Harnessing the anti-cancer natural product nimbolide for targeted protein degradation. Nat. Chem. Biol. (2019-07)

WeissEmily, et al. Generation of antibody-based therapeutics targeting the Idiotype of B-cell Malignancies. Antib Ther. (2019-01)

WardCarl C, et al. Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chem. Biol. (2019)

Nerea Ugidos, et al. Interactome of ANKRD55, an autoimmune disease risk protein with unknown function. frontiersin. (2019)

Wu J, et al. Cryo-EM Structure of the Human Ribonuclease P Holoenzyme. Cell. (2018-11)

Morran MP, et al. Insulin receptor based lymphocyte trafficking in the progression of type 1 diabetes. J Biol Methods. (2018)

Liu T, et al. RBFOX3 Promotes Tumor Growth and Progression via hTERT Signaling and Predicts a Poor Prognosis in Hepatocellular Carcinoma. Theranostics. (2017-07)

Wang X,, Paucek RD,, Gooding AR,, Brown ZZ, Ge EJ, Muir TW, Cech TR,., et al. Molecular analysis of PRC2 recruitment to DNA in chromatin and its inhibition by RNA. Nat Struct Mol Biol. (2017)

de KreukBart-Jan, et al. Heart of glass anchors Rasip1 at endothelial cell-cell junctions to support vascular integrity. Elife. (2016)

ShresthaPravesh, et al. Cloning, Purification, and Characterization of Recombinant Human Extracellular Superoxide Dismutase in SF9 Insect Cells. Mol. Cells. (2016)

You can visit "Citations Database" for more citations.



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Cat. No. L00432
Size Price
1 ml Resin (Total 2 ml) $281.00
5 ml Resin (Total 10 ml) $663.00
10 ml Resin (Total 20 ml) $1193.00
25 ml Resin (Total 50 ml) $2591.00