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His Tag ELISA Detection Kit

Concentration gradient represents X, OD450 value represents Y, all X values will be transformed to Log(X) to create the standard curve which was calculated by 4PL (Figure 1). The operator can get log values of sample concentration by inputting the OD450 value of unknown samples. By formula X=10X, sample concentration can be calculated.

His Tag ELISA Detection Kit

His Tag ELISA Detection Kit

GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged proteins.
L00436
$298.00

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Note

L00436 interferes with IgG in mouse/rat serum and is not suitable for detecting mouse/rat serum samples.

Overview
Product Description GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged proteins. There are several potential usages:
●Quickly identify the presence of His-tagged proteins in samples.
●Optimize protein expression by monitoring the His-tagged proteins level.
●High throughput screening of stable cell lines expressing His-tagged proteins.

Product Introduction
Detection Range 1 ng/ml~729 ng/ml
Sensitivity 1 ng/ml His-tagged proteins
Test Samples N-terminal/C-terminal/internal His-tagged proteins4 x His/5 x His/6 x His-tagged proteins
Mammalian, yeast and bacteria cell lysates or cell supernatant
Conveniency Provide all reagents required for test
Complete test within 1.5 hours
Reagents compatibility Tolerable with various reagents at certain concentration
Storage The unopened kit is stable for at least 12 months if stored at 2-8 °C and the opened kit may be stable for up to 1 month at 2-8 °C. Do not freeze the kit.
Kit Contents
Component Quantity Part. No
His Tag Plate 1 plate (8 wells × 12 strips) 436-80
Anti-His Monoclonal Antibody 6 ml 436-20
Antibody Tracer 12 ml 436-30
His Tag Standards (0,1,3,9,27,81,243,729 ng/ml) 1 ml per each standard 436-11,12,13,14,15,16,17,18
Assay Diluent 60 ml 436-60
20 × Wash Solution 40 ml B-A1-70
TMB Solution 12 ml B-A1-40
Stop Solution 6 ml B-A1-50
His Tag Standard Stock (10 μg/ml) 500 μl 436-10
Plate Sealer 2 N/A

Examples
  • His Tag ELISA Detection Kit
    • His Tag ELISA Detection Kit

    Concentration gradient represents X, OD450 value represents Y, all X values will be transformed to Log(X) to create the standard curve which was calculated by 4PL (Figure 1). The operator can get log values of sample concentration by inputting the OD450 value of unknown samples. By formula X=10X, sample concentration can be calculated.

  • His Tag ELISA Detection Kit
    • His Tag ELISA Detection Kit


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.