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SDS-PAGE analysis of protein purified by Anti-His Affinity Resin or Ni-NTA Agarose.Test result demonstrated that the Anti-His Affinity Resin can purify the His-tagged proteins with high purity from different samples in which Ni-NTA Agarose had failed.
SDS-PAGE analysis of protein eluted from Anti-His Affinity Resin.M. Marker1. His-tagged protein using the acid elution procedure2. His-tagged protein using the alkaline elution procedure3. His-tagged protein by competitive elution procedureTest result demonstrated that the His-tagged protein can be eluted by three different eluents.
45-165 μm, averagely 90 μm
THETM His Tag Antibody, mAb, Mouse (Cat.No A00186)
More than 0.6 mg of His-tagged protein per mL settled resin
N-terminal/C-terminal/internal/4 x His/5 x His/6 x His-tagged protein
Stable at 2-8 °C for 1 year
2-8 °C. Avoid freezing the resin
Generally the resin can be recycled at least 4 times. If maintained properly, the resin may be recycled 10 times with minor binding capability loss
Acid, alkaline and peptide competitive elutions
Compatible with mostly used reagents at certain concentrations
Lot-to-lot consistency proved by strict production procedure
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