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Catalog Products » Protein Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » Anti-His Affinity Resin

Anti-His Affinity Resin

Anti-His Affinity Resin

SDS-PAGE analysis of protein purified by Anti-His Affinity Resin or Ni-NTA Agarose.
Test result demonstrated that the Anti-His Affinity Resin can purify the His-tagged proteins with high purity from
different samples in which Ni-NTA Agarose had failed.

Anti-His Affinity Resin

SDS-PAGE analysis of protein eluted from Anti-His Affinity Resin.
M. Marker
1. His-tagged protein using the acid elution procedure
2. His-tagged protein using the alkaline elution procedure
3. His-tagged protein by competitive elution procedure
Test result demonstrated that the His-tagged protein can be eluted by three different eluents.


Description GenScript Anti-His Affinity Resin is designed for purification of His-tagged protein. It is composed of agarose and conjugated THETM anti-His monoclonal antibody. His-tagged protein in the crude samples can bind to the anti-His monoclonal antibodies coupled with the agarose, and the nonspecific proteins can be eliminated by washing. As a result, the His-tagged protein will finally be eluted with high recovery. Moreover, this product can be used for immunoprecipitation of His-tagged protein.
Key Features
Characteristics:

Features

Specifications

Beads structure

4% agarose

Beads size

45-165 μm, averagely 90 μm

Coupled antibody

THETM His Tag Antibody, mAb, Mouse (Cat.No A00186)

Binding capacity

More than 0.6 mg of His-tagged protein per mL settled resin

Specificity

N-terminal/C-terminal/internal/4 x His/5 x His/6 x His-tagged protein

Stability

Stable at 2-8 °C for 1 year

Storage

2-8 °C. Avoid freezing the resin

Repeat usage

Generally the resin can be recycled at least 4 times. If maintained properly, the resin may be recycled 10 times with minor binding capability loss

Elution methods

Acid, alkaline and peptide competitive elutions

Reagents compatibility

Compatible with mostly used reagents at certain concentrations

Consistency

Lot-to-lot consistency proved by strict production procedure


Cat. No.Product Name Volume supplied Resin content
L00439-1Anti-His Affinity Resin 1 ml2 ml50% slurry
L00439-5Anti-His Affinity Resin 5 ml10 ml50% slurry
Anti-His Affinity Resin
Anti-His Affinity Resin

SDS-PAGE analysis of protein purified by Anti-His Affinity Resin or Ni-NTA Agarose.
Test result demonstrated that the Anti-His Affinity Resin can purify the His-tagged proteins with high purity from
different samples in which Ni-NTA Agarose had failed.

Anti-His Affinity Resin
Anti-His Affinity Resin

SDS-PAGE analysis of protein eluted from Anti-His Affinity Resin.
M. Marker
1. His-tagged protein using the acid elution procedure
2. His-tagged protein using the alkaline elution procedure
3. His-tagged protein by competitive elution procedure
Test result demonstrated that the His-tagged protein can be eluted by three different eluents.


Cat. No.
Product Name
Size
Price
Quantity
A00174-40 His-tag Antibody, pAb, Rabbit
40 ug
$99.00
A00186-100 THETM His Tag Antibody, mAb, Mouse
100 ug
$375.00
A00205-40 His-tag antibody [Biotin], pAb, Rabbit
40 ug
$99.00
A00612 THETM His Tag Antibody [HRP], mAb, Mouse
100 ug
$410.00
A01620 THETM His Tag Antibody [FITC], mAb, Mouse
100 ug
$410.00
L00436 His Tag ELISA Detection Kit
1 kit
$298.00
L00439-5 Anti-His Affinity Resin
5 ml
$1599.00
L00440c His Tag Antibody Plate (clear, 8x12 strips)
5 plates
$179.00
L00440b His Tag Antibody Plate (black, 96 wells, nonremovable)
5 plates
$179.00
A01800-100 THETM His Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$450.00
A01802-100 THETM His Tag Antibody [iFluor 647], mAb, Mouse
100 ug
$450.00
 


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Cat. No. L00439
Size
Price
5 ml $1599.00
1 ml $399.00
24 hour
 
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