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Catalog Products » Protein Isolation and Analysis » Protein Detection and Immunoassay » ELISA Detection Kits » cAMP ELISA Detection Kit

cAMP ELISA Detection Kit

Description
Kit Description

GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP (Adenosine 3',5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cAMP is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5'-AMP by the actions of phosphodiesterases.

The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.
Key Features

Key Features

  • Sensitivity: 0.61 pmol/ml
  • Detection Range: 1~243 pmol/ml
  • Easy to use: All reagents and buffers for cAMP test are provided. Complete the test within 3.5 hours.
  • Cross Reactivity: No significant cross-reactivity of similar compounds was found

    Application

    Quantitative detection of cAMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.

    Storage

    The unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for up to 2 weeksat 2-8 °C. Do not freeze the kit.

    Kit Components

    Component

    Quantity

    Anti-IgG Capture Plate

    1 plate (8 wells x 12 strips)

    Anti-cAMP mAb

    12 ml

    HRP-cAMP

    6 ml

    cAMP Standards
    (0, 1, 3, 9, 27, 81, 243 pmol/ml)

    1.5 ml

    cAMP Standard Stock (10 nmol/ml)

    500 μl

    Assay Buffer A

    60 ml

    Assay Buffer B

    1 ml

    20 × Wash Solution

    40 ml

    TMB Substrate

    12 ml

    Stop Solution

    6 ml

    Plate Sealer

    2 pieces

    User Manual

    1 copy

     


  • Storage Store at 4°C


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    Cat. No. L00460
    Size
    Price
    1 plate (8 wells x 12 strips) $287.00