cAMP ELISA Detection Kit

GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.

Price	$297.00
Cat. No.	L00460
Size	1 plate (8 wells x 12 strips) Buy in bulk
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Product Introduction Bottom Notes

Product Introduction

Product Description

Adenosine 3’, 5’ cyclic monophosphate (cAMP) is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. The G-Protein Coupled Receptors (GPCRs) are critical cell surface receptors that can be activated by different ligands, such as neurotransmitters, hormones and ions. The binding of the ligand to its receptor activates the effector adenylyl cyclase generating cAMP product. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5´-AMP by the actions of phosphodiesterases.
GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine. The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB Solution to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Because the concentration of cAMP-HRP is held constant in the assay, the intensity of color is inversely proportional to the cAMP concentration in the sample and standards. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.

Detection Range

1 ~243 pmol/ml

Sensitivity

0.61 pmol/ml

Test Samples

Serum, plasma, saliva, cell culture supernatant, and urine

Conveniency

All reagents and buffers for cAMP test are provided
Complete the test within 3.5 hours

Storage

The unopened kit is stable for 12 months if stored at 2-8 °C, and the opened kit is stable for 2 weeks at 2-8 °C. Do not freeze the kit.

Kit Contents

Components	Quantity	Part.No
Anti-IgG Capture Plate	1 plate (8 wells x 12 strips)	460-80
Anti-cAMP mAb	12 ml	460-20
HRP-cAMP	6 ml	460-30
cAMP Standards (0, 1, 3, 9, 27, 81, 243 pmol/ml)	1.5 ml	460-11, 12, 13, 14, 15, 16,17
cAMP Standard Stock (10 nmol/ml)	500 μl	460-10
Assay Buffer A	60 ml	460-60
Assay Buffer B	1 ml	460-90
20× Wash Solution	40 ml	B-A1-70
TMB Solution	12 ml	B-A1-40
Stop Solution	6 ml	B-A1-50
Plate Sealer	2 pieces	N/A

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Citations

1. Hong Cao, et al. O-GlcNAc transferase affects the signal transduction of β1 adrenoceptor in adult rat cardiomyocytes by increasing the O-GlcNAcylation of β1 adrenoceptor. Biochem Biophys Res Commun. (2020-05)
2. Touhara KK, et al. Molecular basis of signaling specificity between GIRK channels and GPCRs. Elife. (2018)