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Catalog Products » Protein Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » Protein G Resin FF

Protein G Resin FF


Description GenScript Protein G Resin FF is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin FF is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein G Resin FF.
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.

Cat. No.Product Name Volume supplied Resin content
L00664-5Protein G Resin FF (5ml)10ml50% slurry
L00664-25Protein G Resin FF (25ml)50ml50% slurry
L00664-100Protein G Resin FF (100ml)200ml50% slurry


Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.

Characteristics
LigandRecombinant Streptococcal protein G lacking the albumin-binding sites expressed in E. coli
Number of IgG binding sites per ligand3
M.W. of ligandApproximately 22 kDa
PI of ligand4.69
Degree of substitutionApproximately 2 mg protein G/ml settled resin
Static binding capacity≥ 20 mg human IgG/ml settled resin
Matrix spherical4% highly cross-linked agarose
Average particle size90 μm (45-165 μm)
Recommended flow rate 50-300cm/h
Storage solution20% ethanol
Storage and Stability18 months when stored unopened at 2-8 °C
Storage Store in 20% ethanol at 2°C to 8°C. Do not freeze.



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Cat. No. L00664
Size
Price
5 ml $416.00
25 ml $1473.00
100 ml $2209.00
24 hour
 
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