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Catalog Products » Protein Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » High Affinity Ni-Charged Resin FF

High Affinity Ni-Charged Resin FF


Description GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF the excellent choice for high performance purification of polyhistidine-tagged proteins.

Cat. No.Product Name Volume supplied Resin content
L00666-5High Affinity Ni-Charged Resin FF 5 ml10 ml50% slurry
L00666-25High Affinity Ni-Charged Resin FF 25 ml50 ml50% slurry
L00666-100High Affinity Ni-Charged Resin FF 100 ml200 ml50% slurry


Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.

Characteristics
Binding capacity>40 mg of histidine-tagged protein /ml settled resin
Matrix spherical6% highly cross-linked agarose
Average particle size90 μm (45-165 μm)
Storage solution20% ethanol
Storage and Stability18 months when stored unopened at 2-8 °C

Reagents Compatible
DenaturantsDetergentsReducing agentsSalts Others
6 M Gu·HCl2% Triton X-100 20 mM β-ME4 M MgCl250% glycerol
8 M Urea 2% Tween 20 1 mM DTT 5 mM CaCl220% ethanol
1% CHAPS2 M NaCl1 mM EDTA
Storage Store in 20% ethanol at 2°C to 8°C. Do not freeze.



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Cat. No. L00666
Size
Price
5 ml $62.00
25 ml $253.00
100 ml $849.00
24 hour
 
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