Protein G MagBeads MX

GenScript Protein G MagBeads MX are superparamagnetic beads of average 40 μm in diameter, covalently coated with recombinant Protein G. The beads are supplied as 25% slurry in 20% ethanol. The Protein G MagBeads MX have a binding capacity of more than 25 mg Human IgG per 1 ml settled beads (e.g. 4 ml 25% slurry).

Protein G is a genetically engineered protein (MW≈22 kDa) that combines the IgG binding sites of both Protein A and Protein G. 6×His-tag was attached to its N-terminal to facilitate the purification. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.