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Catalog Products » Protein Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » Protein A Resin FF Prepacked Column

Protein A Resin FF Prepacked Column


Description GenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF Prepacked Column.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.

Cat. No.Product Name Volume supplied Resin content
L00680-12Protein A Resin FF (1mlx2)1mlx250% slurry
L00680-51Protein A Resin FF (5mlx1)5mlx150% slurry
L00680-55Protein A Resin FF (5mlx5)5mlx550% slurry

Characteristics
LigandRecombinant Streptococcal protein A expressed in E. coli
Number of IgG binding sites per ligand5
M.W. of ligandApproximately 34 kDa
PI of ligand5.17
Degree of substitutionApproximately 5 mg protein A / ml settled resin
Static binding capacity> 40 mg human IgG/ ml settled resin
Matrix componentAgarose, 4% highly cross-linked
Average particle size90 μm (45-165 μm)
Storage solution20% ethanol
Storage conditions2-8 °C
Shelf life18 months when stored unopened

Column compatibility
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).
Storage Store in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.



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Cat. No. L00680
Size
Price
5 ml x 5 $2083.00
5 ml x 1 $465.00
1 ml x 2 $251.00
24 hour
 
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