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High Affinity Ni-Charged Resin FF Prepacked Column

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Overview

Description

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.

Cat. No.	Product Name	Volume supplied	Resin content
L00683-12	High Affinity Ni-charged Resin FF prepacked column (1mlx2)	1mlx2	prepacked column
L00683-51	High Affinity Ni-charged Resin FF prepacked column (5mlx1)	5mlx1
L00683-55	High Affinity Ni-charged Resin FF prepacked column (5mlx5)	5mlx5

Storage Store in 20% ethanol at 2°C to 8°C. Do not freeze.

Characteristics

Binding capacity	>40 mg of histidine-tagged protein /ml settled resin
Matrix spherical	6% highly cross-linked agarose
Average particle size	90 μm (45-165 μm)
Storage solution	20% ethanol
Storage and Stability	18 months when stored unopened at 2-8 °C

Column compatibility
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

Reagents Compatible

Denaturants	Detergents	Reducing agents	Salts	Others
6 M Gu·HCl	2% Triton X-100	20 mM β-ME	4 M MgCl₂	50% glycerol
8 M Urea	2% Tween 20	1 mM DTT	5 mM CaCl₂	20% ethanol
	1% CHAPS		2 M NaCl	1 mM EDTA