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Catalog Products » Protein Isolation and Analysis » Protein Isolation and Purification » Protein Purification Resins » High Affinity Ni-Charged Resin FF Prepacked Column

High Affinity Ni-Charged Resin FF Prepacked Column


Description GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.

Cat. No.Product Name Volume supplied Resin content
L00683-12High Affinity Ni-charged Resin FF prepacked column (1mlx2)1mlx2prepacked column
L00683-51High Affinity Ni-charged Resin FF prepacked column (5mlx1)5mlx1
L00683-55High Affinity Ni-charged Resin FF prepacked column (5mlx5)5mlx5

Storage Store in 20% ethanol at 2°C to 8°C. Do not freeze.

Characteristics
Binding capacity>40 mg of histidine-tagged protein /ml settled resin
Matrix spherical6% highly cross-linked agarose
Average particle size90 μm (45-165 μm)
Storage solution20% ethanol
Storage and Stability18 months when stored unopened at 2-8 °C


Column compatibility
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

Reagents Compatible
DenaturantsDetergentsReducing agentsSalts Others
6 M Gu·HCl2% Triton X-100 20 mM β-ME4 M MgCl250% glycerol
8 M Urea 2% Tween 20 1 mM DTT 5 mM CaCl220% ethanol
1% CHAPS2 M NaCl1 mM EDTA
Storage Store in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.



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Cat. No. L00683
Size
Price
5 ml x 5 $448.00
5 ml x 1 $112.00
1 ml x 2 $65.00
 
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