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Ni-IDA Resin FF Prepacked Column

Ni-IDA Resin FF Prepacked Column

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GenScript Ni-IDA Resin FF is a 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by three coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. Ni-IDA Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. Ni-IDA Resin FF Prepacked Column is an excellent choice for high performance purification of polyhistidine-tagged proteins.

Cat. No.Product Name Volume supplied Resin content
L00684-12Ni-IDA Resin FF prepacked column (1mlx2)1mlx2prepacked column
L00684-51Ni-IDA Resin FF prepacked column (5mlx1)5mlx1
L00684-55Ni-IDA Resin FF prepacked column (5mlx5)5mlx5

Binding capacity>40 mg of histidine-tagged protein /ml settled resin
Matrix spherical6% highly cross-linked agarose
Average particle size90 μm (45-165 μm)
Storage solution20% ethanol
Storage and Stability18 months when stored unopened at 2-8 °C

Column compatibility
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

Storage & Stability Store in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.


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