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AmMag™ Protein A Magnetic Beads

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Overview

Description

GenScript AmMagᵀᴹ Protein A Magnetic Beads are super paramagnetic beads with average diameter of 50 μm, covalently coated with alkali tolerant protein A. Protein A is a bacterial cell wall protein isolated from Staphylococcus aureus, which binds to mammalian IgG’s mainly through the Fc regions. The alkali tolerant protein A not only retains the specific binding capacity to Fc region of immunoglobulin molecules, but additionally tolerates alkaline conditions and can withstand rigorous cleaning procedures with 0.1 M NaOH.
The beads are supplied as 25% slurry in 20% ethanol. The AmMagᵀᴹ protein A Magnetic Beads have a binding capacity of 40 mg human IgG per 1 ml settled beads. They can be reused upon washing with 0.1 M NaOH.
The sample containing antibody is added to the AmMagTM Protein A Magnetic Beads and incubated on a shaker for a short time for the antibody to bind to the beads. The antibody bound to the beads can be eluted off of the beads using acidic elution facilitated by a magnetic separation device. Magnetic separation eliminates the need for centrifugation, minimizes the loss of sample and makes the process more user friendly.

Key Features

Quick and convenient separation accomplished by magnetic separation.
High binding capacity, more than 40 mg human IgG/ml settled beads.
Extremely low nonspecific binding.
Optimized homogeneous alkaline-tolerant recombinant protein A ligand.