Description | GenScript AmMagᵀᴹ Protein A Magnetic Beads are super paramagnetic beads with average diameter of 50 μm, covalently coated with alkali tolerant protein A. Protein A is a bacterial cell wall protein isolated from Staphylococcus aureus, which binds to mammalian IgG’s mainly through the Fc regions. The alkali tolerant protein A not only retains the specific binding capacity to Fc region of immunoglobulin molecules, but additionally tolerates alkaline conditions and can withstand rigorous cleaning procedures with 0.1 M NaOH.
The beads are supplied as 25% slurry in 20% ethanol. The AmMagᵀᴹ protein A Magnetic Beads have a binding capacity of 40 mg human IgG per 1 ml settled beads. They can be reused upon washing with 0.1 M NaOH. The sample containing antibody is added to the AmMagTM Protein A Magnetic Beads and incubated on a shaker for a short time for the antibody to bind to the beads. The antibody bound to the beads can be eluted off of the beads using acidic elution facilitated by a magnetic separation device. Magnetic separation eliminates the need for centrifugation, minimizes the loss of sample and makes the process more user friendly. |
Key Features | Quick and convenient separation accomplished by magnetic separation. High binding capacity, more than 40 mg human IgG/ml settled beads. Extremely low nonspecific binding. Optimized homogeneous alkaline-tolerant recombinant protein A ligand. |
Storage | This product is stable until the expiration date stated on the COA, when stored unopened at 2–8°C. Do NOT freeze the product. |
Cat. No. | Product Name | Quantity | Price |
---|---|---|---|
L00722 |
AmMag™ MR-mini magnetic rack - 1 pcs/unit |
$299.00 |
|
L00723 |
AmMag™ MR magnetic rack - 1 pcs/unit |
$1399.00 |
|
L00743 |
AmMag™ Box - 1 kit |
$2399.00 |
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