|Description||GenScript's AmMag Ni Magnetic Beads are optimized for quick and efficient purification of polyhistidine-tagged proteins under native as well as denaturing conditions. The beads use a nickel-charged TED (tris-carboxymethyl ethylene diamine) ligand to purify His-tagged proteins. The beads are resistant to chelators such as EDTA and DTT, and can be regenerated using 1M NaOH. If used without chelator in the sample, they can be regenerated and reused for over 100 times. These superparamagnetic beads are about 70 μm in diameter and their strong magnetic properties allow them to attach very fast to the magnet. The AmMag Ni-charged beads are chelator resistant (up to 100mM EDTA and 20mM DTT). These magnetic beads enable the purification to be performed even with a very high concentration of chelator in the sample, but in such situation, the re-use of beads is impacted. The beads are supplied as 25% slurry in 20% ethanol.|
|Key Features||Quick and convenient separation accomplished by magnetic force.
High binding capacity with 10mg of His-tagged protein/ml settled beads.
The AmMag Ni magnetic beads can be regenerated and reused up to 100 times in absence of chelators.
Extremely low nonspecific binding.
Table 1 Reagents Compatible with AmMag Ni Magnetic Beads
|6 M Gu·HCl||2% Triton X-100||20 mM β-ME||4 M MgCl 2||50% glycerol|
|8 M Urea||2% Tween 20||20 mM DTT||5 mM CaCl 2||20% ethanol|
|0.1 M HCL||1% CHAPS||100 mM EDTA||2 M NaCl|
|1 M NaOH||0.5 M Imidazole|
|Storage||Store at 4°C|
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