|Description||The GenScript SARS-CoV-2 NP&RBD Total Antibody ELISA Detection Kit is intended for the determination of total antibodies (including IgA, IgM and IgG) in human serum or plasma against SARS-CoV-2 Spike NP&RBD recombinant antigen.
The GenScript SARS-CoV-2 NP&RBD Total Antibody ELISA Detection Kit is an indirect ELISA detection tool, which can be used for evaluation of total antibodies (including IgA, IgM and IgG) against SARS-CoV-2 N protein and S1-RBD in human samples.
When the positive control and a specimen are added to the capture plate, the positive control and anti-SARS-CoV-2 N protein and S1-RBD antibodies in a specimen can be captured on the plate. Other unbound molecules are removed by the washing steps. Then, detection antibody is added to the plate to detect specific anti-SARS-CoV-2 antibodies. The detection antibody is a HRP conjugated antibody, and it is an isotype independent secondary antibody recognizing human IgA, IgM, and IgG, etc. After washing steps, TMB solution is added and the color turns blue. The reaction is stopped by adding stop solution and the color turns yellow, which can be read at 450 nm by a microtiter plate reader. The absorbance of the sample dependents on the titer of the anti-NP&RBD antibodies.
Intra-assay: One known level of control was spiked into sample buffer as a test sample. The sample was tested 10 times on the same plate to evaluate intra-assay precision of the kit. Intra-assay variation of this kit is less than or equal to 10%.
Inter-assay: One known level of control was spiked into sample buffer as a test sample. The sample was tested on 3 plates which were randomly selected from 3 different lots to evaluate inter-assay precision of the kit. Inter-assay variation of this kit is less than or equal to 15%.
|Storage||The unopened kit is stable for at least 12 months from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.|
|Note||The operator should read technical manual carefully before using this product.|
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