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AAV2 Titer Capsid ELISA Kit

GenScript’s AAV2 Titer Capsid ELISA Kit (Cat. No. L00942) is an Sandwich ELISA designed for quantitively measuring the titer of any AAV2 in supernatant. This kit makes AAV2 titration easy, accurate and reproducible for GCT applications.

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Product Description GenScript AAV2 Titer Capsid ELISA Kit (Cat. No. L00942) is a Sandwich ELISA Kit that is designed for quantitatively measuring the total capsid titer of adeno-associated virus serotype 2 (AAV2) in testing samples during viral vector production. The total capsid titer stands for the amount of all fully assembled viral capsids, including full and empty AAV2 capsids. This ELISA kit provides an easy, accurate, and reproducible method for AAV2 titration.
Adeno-associated viruses (AAV) are now widely-used viral vectors for gene therapy, with AAV2 being the most extensively studied serotype. When using AAV2 as the viral vector in gene therapy, determination of the total capsid titer of the purified AAV2 particle product is an essential characterization procedure, and represents a critical step in clinical applications.

Product Introduction
Linearity Range 4.8×107 to 2.5×109 Capsids/mL
Sensitivity LoB: 1.31×107 Capsids/mL
LoD: 2.08×107 Capsids/mL
LoQ: 2.15×107 Capsids/mL
Precision Intra-assay: CV ≤ 10%;
Inter-assay: CV ≤ 15%
Assay Temperature 37°C
Kit Contents
Component Quantity/Size Part No.
Capture Plate 1 plate G1-80
AAV2 Control 2 vials (Specified on product label) G1-10
Biotin Anti-AAV2 Antibody 1 bottle (12 mL) G1-20
Streptavidin-HRP 1 bottle (12 mL) E1-30
Sample Dilution Buffer 1 bottle (50 mL) G1-60
TMB Solution 1 bottle (12 mL) A1-40
20× Wash Solution 1 bottle (40 mL) A1-70
Stop Solution 1 bottle (6 mL) A1-50
Plate Sealer 2 pieces N/A
Storage The unopened kit is stable for at least 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

Assay Principle
Assay Principle AAV2 Titer Capsid ELISA Kit is based on the sandwich ELISA method. When standards (AAV2 Control) or samples are added to the capture plate, the AAV2 capsids in the sample can be captured on the plate coated with the capture antibody. Then the detection antibody (Biotin Anti-AAV2 Antibody) conjugated with biotin is added to interact with the AAV2 capsids bound on the plate. Streptavidin-Horseradish Peroxidase conjugate (Streptavidin-HRP) is added to interact with the biotin conjugated detection antibody. After washing steps, 3,3',5,5'-Tetramethylbenzidine solution (TMB Solution) is added resulting in formation of blue color. The reaction is stopped by adding Stop Solution. Application of the Stop Solution results in the color changing from blue to yellow. The intensity of the color can be read at 450 nm and 650 nm by a microplate reader. The quantity of AAV2 capsids in the sample is precisely quantified against an AAV2 standard curve.

Figure 1: Schematic diagram of AAV2 Titer Capsid ELISA Kit

For Research Use Only. Not for Use in Diagnostic Procedures.

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