BSA ELISA Kit

*This product has been discontinued!*

An alternative product for purchase is L00976.

GenScript’s BSA ELISA kit (Cat. No. L00944) is an Sandwich ELISA designed for quantitative measurement of bovine serum albumin (BSA). This kit can be used as a quality control tool during the development of biologics

Cat. No.	L00944
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Product Introduction Assay Principle Bottom Notes

Product Introduction

Product Description

GenScript BSA ELISA kit (Cat. No. L00944) is a Sandwich ELISA Kit that is designed for quantitative measurement of bovine serum albumin (BSA) in testing samples. These outstanding features make it a great solution for routine BSA impurity detection, quality control and lot release, as well as purification process optimization.
Most commercial serum-free media are formulated with various biological macromolecules that can replace the function of serum, such as bovine serum albumin (BSA), transferrin, and insulin. The use of serum-free media can greatly reduce the amount of potential impurities, but the final product serving therapeutic purpose should be highly purified and quality controlled. Hence, it is necessary to test for trace media impurities, and the residual concentration of BSA is usually an important indicator for quality control in production.

Linearity Range

3 to 64 ng/mL

Sensitivity

LoD: 0.5 ng/mL
LoQ: 1 ng/mL

Precision

Intra-assay: CV ≤ 10%;
Inter-assay: CV ≤ 15%

Assay Temperature

37°C

Storage

The unopened kit is stable for at least 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

Kit Contents

Component	Quantity/Size	Part No.
Capture Plate	1 plate	F1-80
Standard Stock	1 vial (50 μL)	F1-10
Biotin Anti-BSA Antibody	1 bottle (12 mL)	F1-20
Streptavidin-HRP	1 bottle (12 mL)	E1-30
Sample Dilution Buffer	1 bottle (12 mL)	F1-60
TMB Solution	1 bottle (12 mL)	A1-40
20× Wash Solution (2% Tween-20)	1 bottle (40 mL)	F1-70
Stop Solution	1 bottle (6 mL)	A1-50
Plate Sealer	2 pieces	N/A

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Assay Principle

Assay Principle

BSA ELISA kit utilizes two anti-BSA monoclonal antibodies which bind to different epitopes of BSA. When the sample and the anti-BSA detection antibody conjugated with biotin are added to the plate (Capture Plate) coated with the anti-BSA capture antibody, the capture antibody and the detection antibody (Biotin Anti-BSA Antibody) bind to the BSA in the sample in a sandwich format. Streptavidin-Horseradish Peroxidase conjugate (Streptavidin-HRP) is added to interact with the Biotin Anti-BSA Antibody. After washing steps, 3,3',5,5'-Tetramethylbenzidine solution (TMB Solution) is added resulting in formation of blue color. The reaction is stopped by adding Stop Solution. Application of the Stop Solution results in the color changing from blue to yellow. The intensity of the color can be read at 450 nm and 650 nm by a microplate reader. The quantity of BSA in the sample is precisely quantified against a BSA standard curve.

Figure 1: Schematic diagram of BSA ELISA Kit

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For Research Use Only. Not for Use in Diagnostic Procedures.