Double-stranded RNA (dsRNA) ELISA Kit (J2 based)

Double-stranded RNA(dsRNA) ELISA Kit (J2 based) is a Sandwich ELISA assay with two kinds of the monoclonal antibodies (J2) to sensitively and selectively detect dsRNA structure (larger than 30-40 bp) in mRNA molecules, independent of their nucleotide composition and sequence.

Price	$870.00
Cat. No.	L01020
Size	96 Tests Buy in bulk
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Product Introduction Bottom Notes

Product Introduction

Linearity Range

0.123 to 30 ng/mL

Sensitivity

LLOQ: 0.123 ng/mL
ULOQ: 30 ng/mL

Precision

Intra-assay: CV ≤ 10%
Inter-assay: CV ≤ 15%

Standard

10 µg/mL of dsRNA with m1Ψ modification

Assay Time

2 h 45 min

Storage

The unopened kit is stable for 12 months from the date of manufacture at 2-8°C, and the opened kit is stable for 30 days from the date of opening at 2-8°C.

Kit Contents

Components	Quantity	Part.No
Capture Plate	1 plate	T1-80
Standard Stock	1 vial (25 μL)	T1-10
100×Detection Antibody	2 vials (Lyophilized)	T1-20
10×HRP-Conjugated Antibody	1 vial (1.50 mL)	T1-30
Sample Dilution Buffer	1 bottle (50 mL)	T1-60
Detection Antibody Dilution Buffer	1 bottle (12 mL)	T1-61
HRP-Conjugated Antibody Dilution Buffer	1 bottle (12 mL)	T1-62
20×Wash Solution	1 bottle (60 mL)	B-A1-71
TMB Solution	1 bottle (12 mL)	B-A1-40
Stop Solution	1 bottle (6 mL)	B-A1-50
Plate Sealer	2 pieces	N/A

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Double-stranded RNA (dsRNA) ELISA Kit (J2 Based)

Figure 1. Double-stranded RNA (dsRNA) ELISA Kit standard curve »

Figure 2 & 3. dsRNA analyzed by ELISA and Dot blot »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.