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Antibody sequencing

- Fastest turnaround: as fast as 10 business days

- Lowest prices: starting at $840

Upgraded! Antibody Sequencing Services

Antibody Sequencing Quotation

The antibody sequence information is essential for monoclonal antibodies (mAbs) engineering, function optimization, database banking, and patent application. Our ISO9001 certified antibody sequencing service is your reliable choice to speed up and facilitate therapeutic antibody leads discovery and development. Tailored to meet your particular requirements, option with customized specifications is offered for antibody sequencing services at GenScript.

Antibody Sequencing: Top 3 Mistakes to Avoid

Our expertise helps you avoid common pitfalls when sequencing antibodies: 1) impurity of hybridoma cells, 2) mutations introduced by PCR, and 3) inaccurate or misaligned full sequences. Learn more from our success to overcome these problems »

  1. Impurity of hybridoma cells: (hybridomas may contain pseudogenes and mRNAs encoding non-functional antibodies, which may be amplified by primer pairs intended to clone the target antibody)
    • GenScript will sequence 5 independent clones for each chain, to ensure accuracy.
  2. Mutations introduced by PCR: During the amplification of the cDNA, the use of degenerate primer sets means there is the chance of mutagenesis.
    • GenScript always uses both 5'- and 3'- RACE (rapid amplification of cDNA ends) to ensure accuracy of amplified products.
    • GenScript always performs full length sequencing of the variable region, which ensures the accuracy of the sequence span, from leader sequence to the start of FR1 region.
  3. Inaccurate or misaligned full sequence (once the individual sequences are obtained for each chain, they must be aligned properly to ensure the full sequence is accurate)
    • GenScript has sequenced over 1,000 mAbs, our expertise ensures the sequence you receive is 100% accurate.

Antibody Sequencing Services

Service Price Upgraded! Turnaround Time Upgraded! Deliverables
Leader sequence & variable domain sequencing $960* 10 business days
  • Final sequence report
  • Sequence data#
  • Alignments of the heavy and light chains#
  • Plasmid containing antibody coding sequence#
Full length antibody sequencing $1,600* 10 business days

*Special discount for batch order
#Upon your request

Customer provides

For sequencing services: You need to submit hybridoma cell samples (>1×106 cells) to GenScript.

GenScript delivers

For sequencing services: Final sequence report with leader sequences and CDR region annotated/ Final sequence report with leader sequences, constant region and CDR region annotated

Sequence data

Alignments of the heavy & light chains and plasmid containing V-region/ full length sequence could be chosen upon your request (SC1408-R/SC1919-R: additional $200 charge).

The client retains all rights to the sequence data and related intellectual property.

mAb sequencing procedure

GenScript offers our customers a one-stop solution for all outsourcing needs, providing comprehensive upstream and downstream services of monoclonal antibody sequencing service.

Antibody Sequencing Quotation

Quotation and Ordering