Antibody sequencing

- Fast turnaround: as soon as 10 business days

- Affordable prices: starting at $840

- Double Assurance: Antigen-rAb binding assay to validate sequence accuracy

Antibody Sequencing Services

Antibody Sequencing Quotation

The antibody sequence information is essential for monoclonal antibodies (mAbs) engineering, function optimization, database banking, and patent application. Our ISO9001 certified antibody sequencing service is your reliable choice to speed up and facilitate therapeutic antibody leads discovery and development. Tailored to meet your particular requirements, option with customized specifications is offered for antibody sequencing services at GenScript.

Antibody Sequencing: Top 3 Mistakes to Avoid

Our expertise helps you avoid common pitfalls when sequencing antibodies: 1) impurity of hybridoma cells, 2) mutations introduced by PCR, and 3) inaccurate or misaligned full sequences. Learn more from our success to overcome these problems »

  1. Impurity of hybridoma cells: (hybridomas may contain pseudogenes and mRNAs encoding non-functional antibodies, which may be amplified by primer pairs intended to clone the target antibody)
    • GenScript will sequence 5 independent clones for each chain, to ensure accuracy.
    • GenScript can also express the whole IgGs in a mammalian expression system and further confirm the antigen-antibody binding performance to double-ensure accuracy.
  2. Mutations introduced by PCR: During the amplification of the cDNA, the use of degenerate primer sets means there is the chance of mutagenesis.
    • GenScript always uses both 5'- and 3'- RACE (rapid amplification of cDNA ends) to ensure accuracy of amplified products.
    • GenScript always performs full length sequencing of the variable region, which ensures the accuracy of the sequence span, from leader sequence to the start of FR1 region.
  3. Inaccurate or misaligned full sequence (once the individual sequences are obtained for each chain, they must be aligned properly to ensure the full sequence is accurate)
    • GenScript has sequenced over 4,000 mAbs, our expertise ensures the sequence you receive is 100% accurate.

Antibody Sequencing Services

Service PriceUpgraded! Turnaround TimeUpgraded! Deliverables
Antibody Sequencing Leader sequence & variable domain sequencing $960* 10 business days
  • Final sequence report with CDR annotated
  • Sequence data#
  • Alignments of the heavy and light chains#
  • Plasmid containing antibody coding sequence#
Full length antibody sequencing $1,600* 10 business days
Antigen-rAb Binding ConfirmationRecommended! 1mg recombinant Ab expression & one-step purification $799@ 18 business days
  • Purified ab
  • QC report&
  • Expression level report
Antigen-rAb binding test by indirect ELISA Free 5 business days ELISA Binding report

*Discount for batch order
#Upon your request
@Applicable to human IgG1, human IgG4 and mouse IgG2.
&QC data includes A280 for Antibody concentration, SDS-PAGE for purity assessment, and Western Blot

Customer provides

For sequencing services: Client will need to submit hybridoma cell samples (>1×106 cells) to GenScript.

For antigen-antibody binding confirmation service: Client will need to submit 20 μg antigen (>85% purity, >0.4mg/ml) in the buffer without SDS, CHAPS, TWEEN, TRITON and PMSF.

GenScript delivers

For sequencing services: Final sequence report with leader sequences and CDR region annotated/ Final sequence report with leader sequences, constant region and CDR region annotated.

Sequence data, alignments of the heavy & light chains (additional $100), plasmid containing antibody coding sequence (additional $100) is available upon your option.

For antigen-antibody binding confirmation service: GenScript delivers 1mg recombinant antibody synthesized from hybridoma sequence, QC, expression level and ELISA binding test report.

The client retains all rights to the sequence data and all related intellectual property.

mAb sequencing procedure

GenScript offers our customers a one-stop solution for all outsourcing needs, providing comprehensive upstream and downstream services of monoclonal antibody sequencing service.

Why do you need Antibody Sequencing?

Why do you need Recombinant Antibody Expression?

Antibody Sequencing Quotation

Quotation and Ordering