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Catalog Products » Other Products » Streptavidin-HRP

Streptavidin-HRP

Description Streptavidin is a protein that has similar binding properties to avidin. It is isolated from streptomyces avidinii. Streptavidin has a molecular weight of 60 KD and has 4 subunits. Each subunit can bind one molecule of biotin. Biotin is a 244 da water soluble vitamin. Streptavidin has an extremely high binding affinity (Kd=10 - 15 ) for biotin.
Streptavidin - HRP (M00091) is a HRP conjugated streptavidin and can be used for analysis of biotinylated molecules.

Concentration 1 mg/ml in PBS (pH 7.4) containing 10 mg/ml BSA and 0.01% thimerosal
Storage The reagent may be stored at 4°C for short periods of one month or less. For longer periods,aliquot and store at -20°C . Avoid repeated freeze/thaw cycles. If slight precipitation occurs upon prolonged storage, clarify the solution by centrifugation before use.

Working concentrations for specific applications should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time. The following dilution ranges are recommended starting points for this product:
Dot blot: 1:2,000-1:10,000
Western blot: 1:2,000-1:20,000
ELISA: 1:50,000-1:200,000
Others: User-optimized


Dreyer AM., et al. Passive Immunoprotection Of Plasmodium Falciparum-Infected Mice Designates The Cyrpa As Candidate Malaria Vaccine Antigen. J Immunol. 2012 Jun 15;188(12):6225-37.

Wang J., et al. PKC promotes the migration of colon cancer cells by regulating the internalization and recycling of integrin αvβ6. Cancer Lett. 2011 Dec 1;311(1):38-47.

Li P., et al. Localized expression pattern of miR-184 in Drosophila. Mol Biol Rep. 2011 Jan;38(1):355-8.

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Cat. No. M00091
Size
Price
1 mg $89.00
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