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Catalog Products » Protein Isolation and Analysis » Protein Detection and Immunoassay » Reagents for Protein Analysis » Biotin-Protein A

Biotin-Protein A

Description Staphylococcal Protein A binds strongly and specifically to the Fc region of immunoglobulin molecules, particularly IgG. The product contains five high-affinity binding sites capable of interacting with the Fc region of IgG in humans, rabbits, pigs, and other species. Biotin-Protein A, which is produced by covalently linking biotin to protein A, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.

Concentration 2 mg/ml in PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide.
Storage Store at 4°C for short periods of time, one month or shorter. Aliquot and store at -20°C for longer periods. Avoid repeated freeze/thaw cycles. Prolonged storage may result in slight precipitation, so clarify the solution by centrifugation before use.

Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration.
Dot blot: 1:5,000-1:50,000
Western blot: 1:5,000-1:50,000
ELISA: 1:50,000-1:500,000
Other: user-optimized.


Cat. No.
Product Name
Price
M00089
HRP-Protein A - 1 mg
$55.00
M00090
HRP-Protein G - 1 mg
$75.00
M00091
Streptavidin-HRP - 1 mg
$89.00
M00097
Biotin-Protein L - 1 mg
$98.00
M00098
HRP-Protein L - 1 mg
$95.00
 


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Cat. No. M00095
Size
Price
2 mg $80.00