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Catalog Products » Protein Analysis & Purification » Protein Detection and Immunoassay » Reagents for Protein Analysis » Biotin-Protein L

Biotin-Protein L

Description Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Biotin-Protein L, which is produced by covalently linking biotin to protein L, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.

Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 1.0 mg/ml.
Concentration 1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.
Storage The product is stable in lyophilized form if stored at -20°C or below. The reconstituted product can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration.
Dot blot: 1:2,000-1:20,000
Western blot: 1:2,000-1:20,000
ELISA: 1:20,000-1:100,000
Other: user-optimized

Cat. No. Product Name Quantity Price
HRP-Protein A - 1 mg
HRP-Protein G - 1 mg
Streptavidin-HRP - 1 mg
Biotin-Protein A - 2 mg
HRP-Protein L - 1 mg
Lanitis E., et al. Redirected antitumor activity of primary human lymphocytes transduced with a fully human anti-mesothelin chimeric receptor. Mol Ther. 2012 Mar;20(3):633-43.

Morgan RA., et al. Recognition of glioma stem cells by genetically modified T cells targeting EGFRvIII and development of adoptive cell therapy for glioma. Hum Gene Ther. 2012 Oct;23(10):1043-53.

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Cat. No. M00097
Size Price
1 mg $98.00