Catalog Products » Stable Cell Lines » Human Recombinant NK1 Tachykinin Receptor Stable Cell Line
CHO-K1/NK1 Stable Cell Line

Figure 1: Substance P-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK1 cells. The cells were loaded with Calcium-4 prior to stimulation with NK1 receptor agonist, Substance P. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Substance P (Mean ± SD, n = 3). The EC50 of Substance P on NK1/CHO-K1 cells was 67 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/NK1 Stable Cell Line

Tachykinins are peptides sharing the common C-terminal amino acid sequence Phe-X-Gly-Leu-Met-NH2. This neuropeptide family is composed of substance P, neurokinin A, and neurokinin B, which are widely distributed in mammalian central and peripheral nervous systems. It plays a role as both a neurotransmitter and a neuromodulator. Their actions are mediated by binding with three distinct receptors, namely, NK1, NK2, and NK3. NK1 has high affinity with substance P. In the CNS, NK1 has been implicated to play a role in regulating neuronal survival and degeneration. In the cardiovascular system, NK1 mediates endothelium-dependent vasodilatation and plasma protein extravasations. In the gastrointestinal system, NK1 receptors mediate intestinal motility, secretion, and vascular functions. SP-NK1 receptor communication is also involved in glioma development and progression. NK1 receptor antagonists may have several therapeutic applications in diseases mediated by tachykinins, such as pulmonary disorders, gut disorders, and the pathophysiology of depression.
M00199
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Description

Tachykinins are peptides sharing the common C-terminal amino acid sequence Phe-X-Gly-Leu-Met-NH2. This neuropeptide family is composed of substance P, neurokinin A, and neurokinin B, which are widely distributed in mammalian central and peripheral nervous systems. It plays a role as both a neurotransmitter and a neuromodulator. Their actions are mediated by binding with three distinct receptors, namely, NK1, NK2, and NK3. NK1 has high affinity with substance P. In the CNS, NK1 has been implicated to play a role in regulating neuronal survival and degeneration. In the cardiovascular system, NK1 mediates endothelium-dependent vasodilatation and plasma protein extravasations. In the gastrointestinal system, NK1 receptors mediate intestinal motility, secretion, and vascular functions. SP-NK1 receptor communication is also involved in glioma development and progression. NK1 receptor antagonists may have several therapeutic applications in diseases mediated by tachykinins, such as pulmonary disorders, gut disorders, and the pathophysiology of depression.

Synonyms

NK1 receptor, SPR, NK1-R, Substance P receptor, Substance P, TAC1R, NK1R, NKIR, NK-1 receptor, NK-1R, Tachykinin 1 receptor, Tachykinin 1 receptor (substance P receptor neurokinin 1 receptor), Tachykinin 1 receptor (substance P receptor neurokinin 1 receptor) see also D4Mgh17 D4Wox19 and D4Mit23, neurokinin 1 receptor, substance-P receptor, tachykinin receptor 1, NK1 receptor, neurokinin receptor 1

Overview
Applications Functional assays for NK1 receptor

Product Introduction
Storage Liquid nitrogen immediately upon delivery
Species Human

Culture Conditions
Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco)

Examples
  • CHO-K1/NK1 Stable Cell Line
  • CHO-K1/NK1 Stable Cell Line

    Figure 1: Substance P-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK1 cells. The cells were loaded with Calcium-4 prior to stimulation with NK1 receptor agonist, Substance P. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Substance P (Mean ± SD, n = 3). The EC50 of Substance P on NK1/CHO-K1 cells was 67 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is the response and starts at Bottom and goes to Top with a sigmoid shape.


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