WB-MASTER Protein Standard

WB-MASTER Protein Standard is designed for convenient protein identification in western blot. This standard consists of seven recombinant proteins with molecular weight of 20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 kDa, 80 kDa and 120 kDa. Each of the proteins contains an IgG binding site that is able to bind to primary or secondary antibodies derived from a wide range of host species. The standard thus enables direct visualization of both the protein marker and users' samples on the same western blot membrane without any additional reagents.

Price	$58.00
Cat. No.	M00521
Size	250 μl Buy in bulk
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Overview Properties Applications

Description

Note

1. Do NOT boil this product.
2. For best results, the loading volume of the marker should be optimized for different experiment conditions (from 2.5 µl to 10 µl).
3. This marker binds to the constant region of IgG, so protein A or protein G fluorephore conjugates cannot be used as the secondary antibody.

Overview

Volume

2.5-10 µl per well, the loading volume of the marker should be optimized for different experiment conditions

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Properties

Calculation	The apparent molecular weights of its seven protein bands are 20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 kDa, 80 kDa and 120 kDa.
Storage	Store at -20°C Avoid repeated freezing and thawing

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Applications

Approximating the size of proteins for Western blots.

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Separated on 4-20 % SDS-PAGE then transferred onto nitrocellulose membrane »

Citations

1. Kevin J. McKernan, et al. Sequence and annotation of 42 cannabis genomes reveals extensive copy number variation in cannabinoid synthesis and pathogen resistance genes. biorxiv. (2020)