CHO-K1/Tim3 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human hepatitis A virus cellular receptor 2 (TIM3) on the surface. The surface expression of TIM3 is validated by FACS analysis.

Price	$8500.00
Cat. No.	M00531
Size	2 vials Buy in bulk
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Product Introduction Culture Conditions Bottom Notes

Product Introduction

Product Description	Recombinant CHO-K1 cells stably overexpress human hepatitis A virus cellular receptor 2 (TIM3) on the surface. The surface expression of TIM3 is validated by FACS analysis.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies).
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma).

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Figure 1. FACS analysis of cell surface expression of human TIM3 on CHO-K1/TIM3 cells. The CHO-K1/TIM3 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human CD366 antibody (Cat. No. 364805, Biolegend). »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.