CHO-K1/mouse 4-1BB Stable Cell Line

Recombinant CHO-K1 cells stably overexpress tumor necrosis factor receptor superfamily member 9 isoform 1 precursor (Mus musculus) on the surface. The surface expression of mouse 4-1BB is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with mouse 4-1BB and evaluating target binding affinity.

Price	$8500.00
Cat. No.	M00568
Size	2 vials Buy in bulk
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Product Introduction Culture Conditions

Product Introduction

Product Description	Recombinant CHO-K1 cells stably overexpress tumor necrosis factor receptor superfamily member 9 isoform 1 precursor (Mus musculus) on the surface. The surface expression of mouse 4-1BB is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with mouse 4-1BB and evaluating target binding affinity.
Culture Properties	Adherent
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

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Figure 1: FACS analysis of cell surface expression of mouse 4-1BB on CHO-K1/mouse 4-1BB cells. The CHO-K1/mouse 4-1BB cells (blue) and the negative control CHO-K1 cells (red) were probed using Mouse IgG control (Whole Molecule), Purified (Cat. No. A01007, GenScript) and [PE] conjugated Goat anti Mouse IgG (H+L) (Cat. No. A10087, GenScript). »