CHO-K1/human CD47 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human CD47 on the surface. The surface expression of CD47 on this cell is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screening antibodies against CD47 or to measure binding affinity between CD47 and anti-CD47 antibodies.

Price	$8500.00
Cat. No.	M00581
Size	2 vials Buy in bulk
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Product Introduction Culture Conditions Bottom Notes

Product Introduction

Product Description	Recombinant CHO-K1 cells stably overexpress human CD47 on the surface. The surface expression of CD47 on this cell is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screening antibodies against CD47 or to measure binding affinity between CD47 and anti-CD47 antibodies.
Culture Properties	Adherent.
Stability	Stability: Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

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Culture Conditions

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies).
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma).

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Figure 1. FACS analysis of cell surface expression of human CD47 on CHO-K1/CD47 cells. The CHO-K1/CD47 cells (Green) and the negative control CHO-K1 cells (Red) were probed using human anti-CD47 antibody (Hu5F9) (Cat. No. B3048, BIOINTRON) and Alexa FluorTM 488 conjugated Alexa FluorTM 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen). »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.