Recombinant CHO-K1 cells
stably overexpress high affinity human Fc fragment of IgG receptor Ia (FcγRI,
CD64) on the cell surface, containing
one alpha chain and two gamma chains. The surface expression of CD64 is
validated by FACS analysis. This stable cell line product is designed for measuring
the binding affinity and stability of the Fc region of antibodies, Fc fusion
proteins, or antibody based biologics that bind with CD64 during biologics research and development.
Cell-based binding assay; use as immunogen for antibody
Codon Optimized from NM_000566.3 (for FCGR1A); Codon
Optimized from NM_004106.2 (for FCER1G); No expressed tags.
of frozen cells (>1×106 per vial in 1 ml)
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received.
The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318, Lonza).
95% complete growth medium,
5% DMSO (Cat. No. D2650, Sigma)
|Complete Growth Medium||
Ham's F-12K (Kaighn's) (Cat.
No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco)
Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10%
FBS (Cat. No.10099-141, Gibco), 300
µg/ml Hygromycin B (Cat. No.10687010, Invitrogen), 8 μg/ml Puromycin (Cat.
FCRI, CD64A, FCGR1, FCGR1A (for Fc Fragment of IgG
receptor Ia); FCER1G, FCRG (for Fc fragment of IgE receptor Ig)
(Cluster of Differentiation 64) is a type of integral membrane glycoprotein, also
known as Fc-gamma receptor 1 (FcγRI). It is composed of two subunits- an alpha
subunit encoded by FCGR1A (Fc Fragment of IgG Receptor Ia), and a gamma subunit
encoded by FCER1G (Fc Fragment of IgE Receptor Ig). It binds monomeric IgG-type
antibodies with high affinity. After binding IgG, CD64 interacts with an
accessory chain known as the common γ chain (γ chain), which possesses an ITAM
motif that is necessary for triggering cellular activation. While CD64 is
constitutively found only on macrophages and monocytes, treatment of
polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64
expression on these cells.
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