Figure 1: FACS analysis of cell surface expression of ACE2 on CHO-K1 cells.
CHO-K1/ACE2 cells (green) and negative control CHO-K1 cells (blue) were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).

Figure 2: FACS analysis of cell surface expression of ACE2 on CHO-K1 cells from different passages.
The CHO-K1/ACE2 cells and the negative control CHO-K1 cells were stained with FITC conjugated SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P6, P11, P16 and P20 represent cell passage numbers.

CHO-K1/ACE2 Stable Cell Line

Recombinant CHO-K1 cells stably express Angiotensin-converting enzyme 2 (ACE2) on the cell surface. The surface expression of ACE2 is validated by FACS analysis. This stable cell line product is designed for cell-based binding assays that measure binding affinity and stability of antibody based biologics binding with ACE2.

Cat. No.	M00771
Size	2 vials : Quote Customized Size
Quantity
Price	$7,500.00


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Overview

Product Description	Recombinant CHO-K1 cells stably express Angiotensin-converting enzyme 2 (ACE2) on the cell surface. The surface expression of ACE2 is validated by FACS analysis. This stable cell line product is designed for cell-based binding assays that measure binding affinity and stability of antibody based biologics binding with ACE2.
Applications	Cell-based binding assay; use as immunogen for antibody development.

Product Introduction

Expressed Gene	Codon Optimized from NM_021804.2; no expressed tags
Target Protein	NP_068576.1
Host Cell	CHO-K1
Quantity	2 vials of frozen cells (>1X10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

Culture Conditions

Culture Properties	Adherent
Freeze Medium	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	F-12K (Cat. # 21127-022, Gibco), 10% FBS (Cat. #10099-141, Gibco)
Culture Medium	F-12K, 10% FBS, 250 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)

Background

Gene Synonyms

Angiotensin-converting enzyme 2; ACEH

Background

Angiotensin-converting enzyme 2 (ACE2), discovered as a homologue of ACE, acts as its physiological counterbalance providing homeostatic regulation of circulating angiotensin II (Ang II) levels. ACE2 is a zinc metalloenzyme and carboxypeptidase located as an ectoenzyme on the surface of endothelial and other cells. While its primary substrate appears to be Ang II, it can hydrolyze a number of other physiological substrates. Additionally, ACE2 functions in other noncatalytic cellular roles including the regulation of intestinal neutral amino acid transport. It also serendipitously acts as the receptor for the severe acute respiratory syndrome virus. Upregulation of ACE2 expression and function is increasingly recognized as a potential therapeutic strategy in hypertension and cardiovascular disease, diabetes, lung injury, and fibrotic disorders. ACE2 is regulated at multiple levels including transcriptional, posttranscriptional (miRNA and epigenetic), and posttranslational through its shedding from the cell surface.

Examples

CHO-K1/ACE2 Stable Cell Line

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