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Catalog Products » Recombined SARS-CoV2 Spike stable cell line

CHO-K1/Spike Stable Cell Line

CHO-K1/Spike Stable Cell Line

FACS analysis of cell surface expression of SARS-CoV-2 Spike protein in CHO-K1/Spike_SARS2 stable cell line by staining with hACE2 protein.

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that cause severe respiratory illness. SARS-CoV-2 Spike Protein is derived from 2019-nCoV, which is composed of a S1 domain and S2 domain. The S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. It is believed that SARS-CoV-2 Spike Protein (RBD) has potential value for the diagnosis of the virus.
To further study the Spike protein of SARS-CoV-2, GenScript developed and successfully established a stable cell line, which can stably overexpress the Spike protein of SARS-CoV-2.

Key Features Single clone
Expression of full length Spike protein including S1 domain and S2 domain (please see the sequence in product protocols)
Validated for stable SARS-CoV-2 spike protein expression and human ACE2 binding
QC Mycoplasma negative
Species SARS-CoV-2
Freeze Medium 95% complete growth medium, 5% DMSO
Culture Medium F-12K, 10% FBS, 8 μg/ml Puromycin

Stability Stable expression of SARS-CoV-2 Spike protein for more than 20 passages
Storage Liquid nitrogen immediately upon receiving

In Vitro binding assay or used as immunogen

  • CHO-K1/Spike Stable Cell Line
  • CHO-K1/Spike Stable Cell Line

    FACS analysis of cell surface expression of SARS-CoV-2 Spike protein in CHO-K1/Spike_SARS2 stable cell line by staining with hACE2 protein.




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Cat. No. M00803
Size Price
2 vials Quote