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Catalog Products » Recombinant Spike_SARS2 Stable Cell Line

HEK293T/Spike Stable Cell Line

Description Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19).
Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells.
Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.
The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N).
The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance.
This glycoprotein mediates attachment of the virus particle and entry into the host cell.
S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
Species SARS-CoV-2
Culture Medium DMEM, 10% FBS, 1 μg/ml Puromycin
Freeze Medium 90% Culture Medium, 10% (V/V) DMSO
QC Mycoplasma negative
Key Features Single clone
Expression of full length Spike protein including S1 domain and S2 domain (please see the sequence in product protocols)
Validated for stable SARS-CoV-2 spike protein expression and human ACE2 binding

Stability Stable expression of SARS-CoV-2 Spike protein for more than 20 passages
Storage Liquid nitrogen immediately upon receiving

In Vitro binding assay or used as immunogen

  • HEK293T/Spike Stable Cell Line
  • HEK293T/Spike Stable Cell Line

    FACS analysis of cell surface expression of SARS-CoV-2 Spike protein in HEK293T/Spike_SARS2 stable cell line by staining with hACE2 protein.

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Cat. No. M00804
Size Price
2 vials Quote