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HEK293T/Spike Stable Cell Line

Recombinant HEK293T cells stably overexpress coronavirus SARS-CoV-2 spike protein on the surface. The surface expression of SARS-CoV-2 spike protein is validated by FACS analysis. This stable cell line product is designed for screening antibodies against SARS-CoV-2 spike protein, as well as for measuring binding affinity and stability of antibody based biologics that bind with spike protein. GenScript also offers spike protein expressing CHO-K1 stable cell line (Cat. No. M00803) for SARS-CoV-2 study.
$7500.00
M00804

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Product Introduction
Product Description Recombinant HEK293T cells stably overexpress coronavirus SARS-CoV-2 spike protein on the surface. The surface expression of SARS-CoV-2 spike protein is validated by FACS analysis. This stable cell line product is designed for screening antibodies against SARS-CoV-2 spike protein, as well as for measuring binding affinity and stability of antibody based biologics that bind with spike protein. GenScript also offers spike protein expressing CHO-K1 stable cell line (Cat. No. M00803) for SARS-CoV-2 study.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
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Culture Conditions
Culture Medium DMEM, 10% FBS, 1 μg/ml puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium Complete Growth Medium: DMEM, 10% FBS.
Freeze Medium-DATA 50% DMEM (Cat. No. 10569, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 5% DMSO (Cat. No. D2650, Sigma)
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HEK293T/Spike Stable Cell Line

Figure 1: FACS analysis of cell surface expression of SARS-CoV-2 spike protein on HEK293T cells. HEK293T/Spike cells (blue) and negative control HEK293T cells (grey) were probed using ACE-2-Fc Chimera, Human (Cat. No. Z03516, GenScript) and Alexa Fluor 647 conjugated Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Cat. No. A-21445, Thermo Fisher).

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.