|Description||Multiple Tag Cell Lysate is whole E. coli cell lysate comprised of about 30% specially designed fusion protein, which act as a positive control for Western blot or Dot blot. The fusion protein has an apparent molecular weight of ~40 kDa on SDS-PAGE and it contains 16 commonly used tags including Trx, T7, HSV, C-Myc, SVS-g, Glu-Glu, V5, E tag, DYKDDDDK, S-tag, HA, KT3, E2, AU1, AU5, and His tag.|
|Volume||Add 200 µl of ddH2O to make the final concentration of 1 µg/µl in 1X SDS Loading Buffer. 0.02 – 0.5 µl of the lysate is recommended for each lane of a minigel. For accurate loading, dilute with 1X SDS Loading Buffer.|
|Concentration||200 µg of whole E. coli cell lysate (lyophilized)|
|Storage||Store at -20°C or below. After reconstitution, aliquot and store at -20°C or below.|
|Positive control for Western blot and Dot blot.
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