cGMP-peroxidase conjugate is a product most suitable for GenScript's THETM Anti cGMP monoclonal antibody (Cat. No. A01508) and Rabbit Anti-cGMP polyclonal antibody (Cat. No. A00615) in immunoassay development for cGMP detection. HRP is conjugated to the C-8 position of cGMP.
cGMP Competitive ELISA 1.ELISA plate was coated with goat anti mouse IgG antibody 2.Anti-cGMP monoclonal antibody (Cat. No. A01508) at appropriate dilution and cGMP standards or testing compounds were added into appropriate reaction wells. 3.After a period of incubation, HRP-cGMP conjugate (Cat.No.M01058) was added followed by proper period of incubation. 4.ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.