• English
  • Sign In
  • Contact Us
×
Catalog Products » Protein Isolation and Analysis » Protein Detection and Immunoassay » Reagents for Protein Analysis » cAMP-HRP

cAMP-HRP

Description cAMP-peroxidase conjugate is a product most suitable for GenScript's THETM Anti cAMP monoclonal antibody (Cat. No. A01509) and Rabbit Anti-cAMP polyclonal antibody (Cat. No. A00614) in immunoassay development for cAMP detection. HRP is conjugated to the C-8 position of cAMP.

Concentration Phopsphate buffered saline, pH 7.4, containing 1% BSA and 0.01% thimerosal.
Storage The conjugate can be stored for up to two years at -20°C or below.
Note Small volumes of the product will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antibody affinity, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this product for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000 - 1:20,000
Other: user-optimized

  • CAMP-HRP
  • CAMP-HRP

    cAMP Competitive ELISA
    1. ELISA plate was coated with goat anti mouse IgG antibody
    2. Anti-cAMP monoclonal antibody (Cat. No. A01509) at appropriate dilution and cAMP standards or testing compounds were added into appropriate reaction wells.
    3. After a period of incubation, cAMP-HRP conjugate (Cat.No.M01059) was added followed by proper period of incubation.
    4. ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature.
    5. Stop the reaction with 1.0 N HCl and read the plate at 450nm.


Cat. No.
Product Name
Price
A01509-100
THETM cAMP Antibody, mAb, Mouse - 100 ug
$330.00
A01509-40
THETM cAMP Antibody, mAb, Mouse - 40 ug
$158.00
A01509-200
THETM cAMP Antibody, mAb, Mouse - 200 ug
$594.00
A00614-40
cAMP Antibody, pAb, Rabbit - 40 μl
$99.00
 
Takayuki Nitta, et al. The Cellular Protein La Functions in Enhancement of Virus Release through Lipid Rafts Facilitated by Murine Leukemia Virus Glycosylated Gag. MBio.2011 Feb 22;2(1):e00341-10.

You can visit "Citations Database" for more citations.





For more documents, please visit "Technical Support".

Cat. No. M01059
Size
Price
200 μl $105.00