cAMP-peroxidase conjugate is a product most suitable for GenScript's THETM Anti cAMP monoclonal antibody (Cat. No. A01509) and Rabbit Anti-cAMP polyclonal antibody (Cat. No. A00614) in immunoassay development for cAMP detection. HRP is conjugated to the C-8 position of cAMP.
cAMP Competitive ELISA 1. ELISA plate was coated with goat anti mouse IgG antibody 2. Anti-cAMP monoclonal antibody (Cat. No. A01509) at appropriate dilution and cAMP standards or testing compounds were added into appropriate reaction wells. 3. After a period of incubation, cAMP-HRP conjugate (Cat.No.M01059) was added followed by proper period of incubation. 4. ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature. 5. Stop the reaction with 1.0 N HCl and read the plate at 450nm.