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In molecular cloning, after the synthesis of cDNA from mRNA molecule templates, a PCR program must be designed to amplify the gene of interest, as well as add additional elements such as restriction sites or detection/purification tags. Intrinsic properties of gene sequences such as high GC content, long stretches of the same polynucleotide, and sequences encoding hairpin loop structures can all hinder PCR efficiency.
Use the troubleshooting guide below to identify the cause of PCR failure and improve PCR efficiency or get your desired gene in the vector you want the easy way with GenEZ™ ORF clones. Start with a search for your gene.
PCR protocol »
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