HPLC purified pegRNA (218 nt): Precise molecular weight

Desalt purified pegRNA (218 nt): Precise molecular weight

HPLC purified pegRNA (218 nt): Purity up to 92.78%

Prime Medicine
I have been using GenScript to order customized critical reagents for an important assay. GenScript can always deliver materials efficiently with good quality. Their technical support is great at addressing any additional questions.
Up to 231 nt for Both Desalt &
HPLC Purified Grades
Precise molecular weight, high purity, excellent batch-to-batch consistency
Proven Editing Efficiency
Editing efficiency over 60%
Modifications to enhance efficiency
One-Stop Solution
PE2/PE3 mRNA available
Validated experiment protocol
RUO to cGMP Capability
Supporting research to IND application & clinical trials
Service | pegRNA (Prime Editing guide RNA) Synthesis Service |
Length | 110-231 nt* *For <110 nt or>200 nt RNA oligo synthesis, please inquire here |
Modification |
|
Purification | Desalt & HPLC purification options (Purity is guaranteed ≥ 85% for HPLC purified grade )* |
Quantity | 2-100 nmol, lyophilized powder. Optional suspension in TE buffer or NF-dH2O also available. For other quantities, please inquire here |
QC Reports | COA and MS report (HPLC report available for HPLC purity grade) |
Production Time | 7 business days (Excluding shipping time) |
* ≥85% purity is guaranteed for HPLC-purified pegRNA 110-200 nt in length. For other lengths, please inquire here.
Need help from an expert?
Length* | Quantity | Desalt Grade | HPLC Grade |
---|---|---|---|
110-150 nt | 2 nmol | $240 | Inquire for pricing |
5 nmol | $330 | ||
10 nmol | $450 | ||
50 nmol | $1100 | ||
100 nmol | $1600 | ||
151-231 nt | 2-100 nmol | Inquire for pricing |
Prime Editing Products: Rapid and Efficient Validation and Optimization of your PE Editing System
Are you looking to validate your prime editing experimental system or optimize for editing efficiency? Try our off-the-shelf Prime Editing products:
(Refer to the protocol here and see validation data in Case 2 here.)
Off the Shelf (OTS) Product | Catalog No. | Purification Methods | Qty | List Price |
---|---|---|---|---|
PE2/PE3 mRNA | SC2346 | / | 0.2mg/ 1mg | $350/ $1300 |
Human HEK3 PegRNA - 141nt | KIOK16 | HPLC | 2 nmol | $149 |
Human HEK3 ePegRNA (3' tevopreQ1) - 218nt | KIOK17 | HPLC | 2 nmol | $199 |
Human HEK3 nicking sgRNA | KIOK18 | HPLC | 2 nmol | $49 |
Human HEK3-F validation primer | KIOK19 | Desalt | 0.5 nmol | $4 |
Human HEK3-R validation primer | KIOK20 | Desalt | 0.5nmol | $4 |
HPLC purified pegRNA (218 nt): Precise molecular weight
Desalt purified pegRNA (218 nt): Precise molecular weight
HPLC purified pegRNA (218 nt): Purity up to 92.78%
Methods: HEK293T cells (0.2 million cells) were transfected with 1 μg PE2/PE3 mRNA, 30pmol nicking sgRNA and 90pmol pegRNA of different lengths (HPLC grade) targeting the HEK3 gene, via electroporation. Genome DNA was then extracted 3 days post-transfection for editing efficiency analysis via Sanger sequencing.
Results: pegRNA with a tevopreQ1 motif achieved editing efficiencies as high as 43.4%, which was significantly higher than that of the pegRNA without the tevopreQ1 motif.
Note: the 218 nt pegRNA is an extended version of 181 nt with a tevopreQ1 motif (37 nt) at the 3’ end. It has been reported that adding the RNA structural motifs evopreQ1 or mpknot to the 3’ end of a pegRNA can protect the 3’ end from exonuclease digestion (Nelson, J. W. et al., 2022).
Methods: 0.2 million HEK293T cells were transfected with different dosages of 141 nt long pegRNA (desalt or HPLC purified grade) targeting the HEK3 gene, via electroporation. Nicking sgRNA was also added in a ratio of 3:1 to pegRNA. PE2/PE3 mRNA was added with increasing dosages from 0.15 μg, 0.25 μg, 0.375 μg, 0.5 μg, 0.75 μg to 1.0 μg. Genome DNA was then extracted 3 days post-transfection for editing efficiency analysis via Sanger sequencing.
Results: Editing efficiencies up to 52.5% for desalt and 63.5% for HPLC-purified pegRNA was achieved. Editing efficiency increased in a dose-dependent manner.
Methods: HEK293T cells (0.2 million cells) were transfected with a 1 μg pCMV-PE2/PE3 plasmid and 45pmol of 141 nt long pegRNA targeting the HEK3 gene, via electroporation. Genome DNA was then extracted 3 days post-transfection for editing efficiency analysis via Sanger sequencing. 15pmol of additional nicking sgRNA was added to direct the prime editor enzyme to nick the non-edited strand in the PE3 system.
Results: GenScript’s HPLC grade and desalt pegRNA achieved comparable editing efficiency to Vendor I at a more cost-effective price.
97 to 103 nt, improving your editing efficiency at a cost-effective price
Learn More40 to 49 nt, with superior quality and editing efficiency up to 98%
Learn MoreGenScript can also synthesize desalt and HPLC purified guide RNA compatible with saCas9, Cas12f/CasMINI, Cas13, and other Cas nuclease variants.
Click here to enter your guide sequence (target sequence + scaffold sequence) and order online.