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Catalog Products » Other Products » Catalog Peptides » All Catalog Peptides » Pep-1 (uncapped)

Pep-1 (uncapped)

Description PEP-1 peptide, which has 21 amino acid residues, is a known carrier peptide that delivers full-length native proteins in vitro and in vivo. Pep-1 peptide, consists of three domains: (1) a hydrophobic tryptophan-rich motif containing five tryptophan residues (KETW WETWWTEW); (2) a hydrophilic lysine-rich domain (KKKRKV) derived from the nuclear localization sequence (NLS) of simian virus 40 (SV-40) large T antigen; and (3) a spacer domain (SQP), separating the two domains mentioned above, containing a proline residue, which improves the flexibility and the integrity of both the hydrophobic and the hydrophilic domains. In standard cell culture conditions, Pep-1 localizes rapidly, in <10 min, to the nucleus of human HS-68, murine NIH-3T3 fibroblasts, or Cos cells. Similar experiments, performed by incubating cells for 30 min at 4°C before transfection, yielded essentially the same result, indicating that Pep-1 internalization is independent of normal endocytosis.
Sequence
{LYS}{GLU}{THR}{TRP}{TRP}{GLU}{THR}{TRP}{TRP}{THR}{GLU}{TRP}
{SER}{GLN}{PRO}{LYS}{LYS}{LYS}{ARG}{LYS}{VAL}
Sequence Shortening KETWWETWWTEWSQPKKKRKV
Molecular Formula C136H195N35O33
Molecular Weight 2848.3

Purity > 95%
Gmp Flag 0
Storage Store at -20°C
Note


Weller K, et al. Biophysical and biological studies of end-group-modified derivatives of Pep-1. Biochemistry. Dec 2005; 44(48):1 5799-5811.

Henriques ST, et al. Re-evaluating the role of strongly charged sequences in amphipathic cell-penetrating peptides: a fluorescence study using Pep-1. FEBS. Lett. Aug 2005; 579(20): 4498-4502.

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Cat. No. RP10130
Size
Price
1 mg $142.00