Substance P, Free Acid

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The conformation of substance P (free acid) (SPOH) has been investigated in dimethylsulfoxide (DMSO), water and dipalmitoylphosphotidylcholine (DPPC) bilayers by two-dimensional NMR and restraint molecular dynamics simulations. The observed NOE patterns for substance P (free acid) in these media are very much different from each other. The conformation of substance P (free acid) in DPPC bilayers is characterized by gamma-bends at Pro4,Gln6and Phe7, which are stabilized by hydrogen bonding between Lys3CO-->Gln5NH, Gln5CO-->Phe7NH and Gln6CO-->Phe8NH, respectively. The absence of biological activity in SPOH has been attributed to the absence of any helix like structure at the central residues and absence of any interresidue interaction with C-terminal OH group, in DPPC bilayers, a feature shown to be an important prerequisite for SP and SP agonists to bind to the NKI tachykinin receptor.

Cat. No.	RP10185
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Overview Properties

Overview

Synonyms	SPOH
Description	Gln⁵NH, Gln⁵CO-->Phe⁷NH and Gln⁶CO-->Phe⁸NH, respectively. The absence of biological activity in SPOH has been attributed to the absence of any helix like structure at the central residues and absence of any interresidue interaction with C-terminal OH group, in DPPC bilayers, a feature shown to be an important prerequisite for SP and SP agonists to bind to the NKI tachykinin receptor. --> The conformation of substance P (free acid) (SPOH) has been investigated in dimethylsulfoxide (DMSO), water and dipalmitoylphosphotidylcholine (DPPC) bilayers by two-dimensional NMR and restraint molecular dynamics simulations. The observed NOE patterns for substance P (free acid) in these media are very much different from each other. The conformation of substance P (free acid) in DPPC bilayers is characterized by gamma-bends at Pro⁴,Gln⁶and Phe⁷, which are stabilized by hydrogen bonding between Lys³CO-->Gln⁵NH, Gln⁵CO-->Phe⁷NH and Gln⁶CO-->Phe⁸NH, respectively. The absence of biological activity in SPOH has been attributed to the absence of any helix like structure at the central residues and absence of any interresidue interaction with C-terminal OH group, in DPPC bilayers, a feature shown to be an important prerequisite for SP and SP agonists to bind to the NKI tachykinin receptor.
Cas No	71977-09-8
Sequence	{ARG}{PRO}{LYS}{PRO}{GLN}{GLN}{PHE}{PHE}{GLY}{LEU}{MET}
Sequence Shortening	RPKPQQFFGLM
Molecular Formula	C₆₃H₉₇N₁₇O₁₄S₁
Molecular Weight	1348.7

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Properties

Purity	> 95%
Form	Lyophilized
Storage	Store at -20°C.

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