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Catalog Products » Other Products » Catalog Peptides » Catalog Peptides - Hot Products » DYKDDDDK Peptide

DYKDDDDK Peptide

Synonyms DYKDDDDK tag;Flag-tag
Description A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.
Sequence
{ASP}{TYR}{LYS}{ASP}{ASP}{ASP}{ASP}{LYS}
Sequence Shortening DYKDDDDK
Molecular Formula C41H60N10O20
Molecular Weight 1012.97

Purity > 95%
Storage Store at -20°C.
Note


Cat. No.
Product Name
Price
A00170-40
DYKDDDDK-tag Antibody, pAb, Rabbit - 40 ug
$99.00
 

Herold MJ, et al. The stability and anti-apoptotic function of A1 are controlled by its C terminus. J Biol Chem. May 2006; 281(19): 13663-13671.

Park JH, Roeder RG. GAS41 Is Required for Repression of the p53 Tumor Suppressor Pathway during Normal Cellular Proliferation. Mol Cell Biol. Jun 2006; 26(11): 4006-4016.

Inoue H., etc. An essential role for chloroplast heat shock protein 90 (Hsp90C) in protein import into chloroplasts. Proc Natl Acad Sci U S A. 2013 Feb;110(8):3173 - 8.

Jacob Verghese and Kevin A. Morano.., etc. A Lysine-Rich Region Within Fungal Bag Domain-Containing Proteins Mediates A Novel Association With Ribosomes. Eukaryot Cell. 2012 Aug;11(8):1003 - 1011.

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Cat. No. RP10586
Size
Price
1 mg $34.00