Ala-Lys-Arg-Arg-Arg-Leu-Ser-Ser-Leu-Arg-Ala
*This product has been discontinued!*
This sequence, AKRRRLSSLRA, is derived from rat hepatic 40S ribosomal protein S6 (229-239). AKRRRLSSLRA is substrate for Rho-kinase II (ROCK II), Km=0.9 μM and for p90 rsk (S6K) in cell extracts (Km=0.18 mM) using phosphocellulose assay. AKRRRLSSLRA peptide phosphorylation by PKS and PKC can be eliminated by the inclusion of PKI and EGTA, respectively, in the assay 2-4.
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